Whole-mount RNA in situ hybridization was performed as described in “Early Development of Xenopus Laevis: A Laboratory Manual” [72 ]. For immunocytochemistry, anti-H3S10Ph antibody (1:500, Active Motif, Carlsbad, CA, USA) and anti-mouse alkaline phosphatase–conjugated (1:2,000, Chemicon/Thermo Fisher Scientific, Waltham, MA, USA) secondary antibody were used. Embryos were photographed with a Leica M205FA stereomicroscope. Signal from H3S10Ph+ cells was counted using ImageJ software. For confocal imaging, anti-H3S10Ph antibody (1:500, Active Motif), anti-x-β-catenin antibody (1:100, Elizabeth Kremmer), and DAPI were used. Embryos were photographed with a Leica TCS SP5II confocal microscope, using Z-stack montage function. Z-stack depth, 30 μm.
Anti h3s10ph antibody
Manufactured by Active Motif
Sourced in United States
The Anti-H3S10Ph antibody is a laboratory reagent that detects the phosphorylation of serine 10 on histone H3. It is used for the identification and quantification of this specific histone modification in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti h3s10ph antibody
1
Whole-mount RNA In Situ Hybridization in Xenopus
2
Xenopus Embryonic Cell Fate Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Whole-mount RNA in situ hybridization was performed as described in "Early Development of Xenopus Laevis: A Laboratory Manual" (Sive, Grainger and Harland, 2000) . For immunocytochemistry anti-H3S10Ph antibody (1:500, Active Motif) and anti-mouse alkaline phosphatase-conjugated (1:2000, Chemicon) secondary antibody was used. Embryos were photographed with a Leica M205FA stereomicroscope. Signal from H3S10Ph+ cells was counted using ImageJ software. For confocal imaging anti-H3S10Ph antibody (1:500, Active Motif), anti-x-β-catenin antibody (1:100, Elizabeth Kremmer) and DAPI were used. Embryos were photographed with a Leica TCS SP5II confocal microscope, using Z-stack montage function. Z-stack depth -30 micron.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!