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Anti caspase 1 clone 5b10

Manufactured by Thermo Fisher Scientific
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Anti-caspase-1 clone 5B10 is a monoclonal antibody that specifically recognizes the human caspase-1 protein. Caspase-1 is a key enzyme involved in the inflammatory response and apoptotic pathways. The antibody can be used for the detection and analysis of caspase-1 expression in various experimental systems.

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Lab products found in correlation

Nupage bis tris gradient gel, by Thermo Fisher Scientific (2 mentions) Nitrocellulose membrane, by Thermo Fisher Scientific (2 mentions) Anti β actin, by Abcam (2 mentions) Odyssey infrared imaging system, by LI COR (2 mentions) Rabbit anti mouse caspase 1 p10 clone m 20, by Santa Cruz Biotechnology (2 mentions) Rabbit anti mouse il 18 clone h 173, by Santa Cruz Biotechnology (2 mentions)

Spelling variants of this product

Caspase-1 (22 citations) Anti-caspase-1 (10 citations)

2 protocols using anti caspase 1 clone 5b10

1

Caspase-1 and IL-18 Detection in Renal Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein detection from renal tissue preserved at −80 oC was performed as previously described60 . Briefly, 100 µg of total protein were resolved in a 4–12% NuPAGE Bis-Tris gradient gel (Invitrogen). Proteins were immobilized onto a nitrocellulose membrane (Invitrogen) and blocked with 10% BSA for 30 minutes at room temperature. Membrane was incubated with anti-caspase-1 clone 5B10 (1:500; eBioscience), rabbit anti-mouse caspase-1 (p10) clone M-20 (1:500, Santa Cruz), rabbit anti-mouse IL-18 clone H-173 (1:500, Santa Cruz) or anti-beta-actin (1:1,000; Abcam ab8227) overnight at 4 °C. Subsequently, membrane was probed with secondary IRDye680 goat anti-rat or IRDye800 goat anti-rabbit antibodies (1:10,000, Li-Cor Biosiences, Lincoln, NE) for 1 hour at room temperature. Proteins were detected using the Li-Cor Odyssey Infrared Imaging System following manufacturer’s instructions (Li-Cor Biosciences).
Lood C., Blanco L.P., Purmalek M.M., Carmona-Rivera C., De Ravin S.S., Smith C.K., Malech H.L., Ledbetter J.A., Elkon K.B, & Kaplan M.J. (2016). Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease. Nature medicine, 22(2), 146-153.
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2

Caspase-1 and IL-18 Detection in Renal Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein detection from renal tissue preserved at −80 oC was performed as previously described60 . Briefly, 100 µg of total protein were resolved in a 4–12% NuPAGE Bis-Tris gradient gel (Invitrogen). Proteins were immobilized onto a nitrocellulose membrane (Invitrogen) and blocked with 10% BSA for 30 minutes at room temperature. Membrane was incubated with anti-caspase-1 clone 5B10 (1:500; eBioscience), rabbit anti-mouse caspase-1 (p10) clone M-20 (1:500, Santa Cruz), rabbit anti-mouse IL-18 clone H-173 (1:500, Santa Cruz) or anti-beta-actin (1:1,000; Abcam ab8227) overnight at 4 °C. Subsequently, membrane was probed with secondary IRDye680 goat anti-rat or IRDye800 goat anti-rabbit antibodies (1:10,000, Li-Cor Biosiences, Lincoln, NE) for 1 hour at room temperature. Proteins were detected using the Li-Cor Odyssey Infrared Imaging System following manufacturer’s instructions (Li-Cor Biosciences).
Lood C., Blanco L.P., Purmalek M.M., Carmona-Rivera C., De Ravin S.S., Smith C.K., Malech H.L., Ledbetter J.A., Elkon K.B, & Kaplan M.J. (2016). Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease. Nature medicine, 22(2), 146-153.
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