Anti gapdh 14c10

For immunoblotting, we employed the following antibodies from Cell Signaling Technology (Frankfurt am Main, Germany): anti-p73 Rabbit mAB (D3G10, #14620), anti-phospho-ERK1/2 (#4370), anti-GAPDH (14C10, #2118), and anti-Snail (#3895), as well as HRP-linked anti-rabbit (#7074) and anti-mouse (#7076) secondary antibodies; anti-HSP90 (F-8, #sc-13119) was purchased from Santa Cruz Biotechnology (Heidelberg, Germany). Recombinant human TGF-β1 (#300-023) was supplied by ReliaTech (Wolfenbüttel, Germany). An siRNA to TAp73 (5′-cgg auu cca gca ugg acg uTT-3′ and 5′-acg ucc aug cug gaa ucc gTT-3′) and a scrambled control siRNA were commercially synthesized by Metabion Int. AG (Planegg, Germany). An siRNA to SMAD4 (#1027415) was purchased from Qiagen (Hilden, Germany). The TAp73α and TAp73β vectors were a kind gift from Drs. Bertrand Joseph and Pinelopi Engskog Vlachos (Stockholm, Sweden).

The following antibodies were purchased from Cell Signaling Technology (Danvers, MA): AKT (rabbit, #9272), p44/42 MAPK (Erk1/2) (3A7) (mouse, #9107), Syk (D3Z1E) XP (rabbit mAb, #13198), beta-tubulin (rabbit, #2146), USP10 (D7A5) (rabbit mAb, #8501) and FES (rabbit, #2736) were used at 1:1000 for immunoblotting, and anti-GAPDH (14C10) (rabbit mAb, #2118) was used at 1:3000.
Flt-3/Flk-2 (C-20) (sc-479) and ubiquitin (P4D1) (sc-8017) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX) and used at 1:1000 for immunoblotting. Anti-pTyr (mouse, clone 4G10) was purchased from MilliporeSigma (Burlington, MA) and was used at 1:1000 for immunoblotting in the presence of 4% BSA. Anti-HAUSP/USP7 antibody (ab4080) was purchased from Abcam (Cambridge, MA) and used at 1:1000 for immunoblotting.