Two-step immunohistochemistry was used in our study. Firstly, tissue sections were treated to retrieval antigen using EDTA buffer; and then tissues were incubated with primary antibody anti- USP15 (1:800, Proteintech) at 4°C overnight. Next sections were incubated with secondary antibody (HRP-labeled anti-mouse antibody, DAKO), then visualized using diaminobenzidine (DAB) and hematoxylin re-dying after washed with PBS. The immunohistochemical results were valuated by pathologists, and scored as follows: negative for 0, “+” for 1, “++” for 2 and “+++” for 3. The positive staining rate was defined according to the proportion of positive stained cancer cells: “Negative” is 0, “1%-20%” for 1, “21%-40%” for 2, “41-60%” for 3, “61-80%” for 4, “81-100%” for 5. Take the product of “dyeing intensity” score and “dyeing positive rate” score as the total score.
Hrp labeled anti mouse antibody
Manufactured by Agilent Technologies
Sourced in Germany
The HRP-labeled anti-mouse antibody is a laboratory reagent used for the detection of mouse proteins in various immunoassay techniques. It consists of an antibody that specifically binds to mouse proteins, conjugated with the enzyme horseradish peroxidase (HRP). This product can be used to develop colorimetric or chemiluminescent signals in applications such as Western blotting, ELISA, and immunohistochemistry, where the presence and abundance of mouse proteins need to be identified and quantified.
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Lab products found in correlation
2 protocols using hrp labeled anti mouse antibody
1
Immunohistochemical Analysis of USP15 Expression
2
Immunoreactivity of Novel mAbs
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Immunoreactivity of the novel mAbs under denaturing conditions was tested by Western-blot analysis. Full length rNheA or the N-terminally deleted NheA fragments as well as supernatants of B. cereus strains were run on 10–15% Minigel (G&E Healthcare, USA) followed by blotting on a PVDF-P membrane (Merck Millipore, Germany) and blocking with 3% casein-PBS. Primary antibodies (2 μg ml-1) were incubated for 1 h at room temperature. After washing, an HRP-labeled anti-mouse antibody (1:2,000, Dako, Germany) was added for 1 h. Chemiluminescence signals induced by addition of Super Signal Western Femto substrate (Pierce, USA) were recorded on a Kodak image station (Eastman Kodak, USA).
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