Bio plex pro mouse cytokine 23 plex panel kit

The cytokine levels of the BALF and serums were quantified using the Bio-plex Pro mouse cytokine 23-plex panel kit (M60009RDPD, Bio-Rad, USA). The measured cytokines were IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, Eotaxin, G-CSF, GM-CSF, IFN-γ, KC, MCP-1, MIP-1α, MIP-1β, RANTES and TNF-α. Multiplex assay was performed on the Bio-Plex 200 system (Bio-Rad, USA) according to manufacturer’s instructions.
In the in vitro BMDM experiments, the cytokines secreted by M1 macrophages (IL-12/23p40, IL-6 and IL-12p70) and M2 macrophages (IL-10) were quantified by ELISA (R&D Systems, USA) following the manufacturer’s protocols.

Cytokine levels of the colon tissue cultures were measured using the Bio-Plex 200 System (Bio-Rad, Hercules, CA, USA) with the Bio-Plex Pro Mouse Cytokine23-Plex Panel kit (Bio-Rad), according to the manufacturer's instructions. Concentration of IL-6 and TNF-α were measured using ELISA kits (BioLegend, Cambridge, UK) according to the manufacturer's instructions.