The cell culture supernatants were transferred to sterile centrifuge tubes, centrifuged at 1000 × g for 10 min at 4°C, and the supernatants were aliquoted into small EP tubes and stored at −20°C until use. The levels of inflammatory factors in supernatants, including TNF-α and IL-6, were determined by an ELISA system (SEKH-0047, SEKH-0013, Solarbio, China). All experiments were performed according to the manufacturer’s protocols.
Sekh 0047
Manufactured by Solarbio
Sourced in China
SEKH-0047 is a laboratory equipment product. It is a compact, portable centrifuge designed for general laboratory use. The centrifuge can accommodate standard 1.5 mL and 2.0 mL microcentrifuge tubes. It operates at a maximum speed of 6,000 rpm and provides a maximum relative centrifugal force of 2,000 x g.
Automatically generated - may contain errors
Lab products found in correlation
Sekh 0013, by Solarbio (2 mentions)
Sekm 0002, by Solarbio (1 mentions)
Sekh 0018, by Solarbio (1 mentions)
Sekm 0007, by Solarbio (1 mentions)
Sekm 0019, by Solarbio (1 mentions)
Sekm 0034, by Solarbio (1 mentions)
Bc0175, by Solarbio (1 mentions)
Bc0205, by Solarbio (1 mentions)
Gsh px a005 1 2, by Nanjing Jiancheng (1 mentions)
4 protocols using sekh 0047
1
Quantifying Inflammatory Cytokines in Cell Culture
2
Cytokine Profiling in Lung Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The levels of IL-6 (SEKM-0007 and SEKH-0013, Solarbio), tumor necrosis factor-α (TNF-α) (SEKM-0034 and SEKH-0047, Solarbio), IL-10 (SEKM-0010 and SEKH-0018, Solarbio), IL-18 (SEKH-0028 and SEKM-0019), and IL-1β (SEKM-0002 and SEKH-0002) in lung tissues and cell cultures were measured according to the instructions.
3
Serum Biomarkers in Intracerebral Hemorrhage
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fifty participants in the control group were drawn fasting peripheral blood in the morning, while 200 ICH participants were drawn peripheral blood at admission. All peripheral blood was centrifuged at room temperature to collect serum, and the serum was frozen in liquid nitrogen for testing. Serum IL-31, C-reactive protein (CRP), and TNF-α were detected using human IL-31 (SEKH-0040, Solarbio), CRP (EH0099, FineTest), and TNF-α (SEKH-0047, Solarbio) enzyme-linked immunoassay kits, respectively.
4
Whole Blood Analysis in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The whole blood routine in mice was examined using ABAXIS (Union City, CA, USA). The collected blood samples were allowed to stand on ice for 30 min, then centrifuged at 3000 rpm for 10 min, and the supernatant was collected. The activities of tumor necrosis factor (TNF-α), superoxide dismutase (SOD), and catalase (CAT) in serum were detected following the instructions of the kits (SEKH-0047, BC0175, BC0205, Solarbio, Beijing, China), respectively. Glutathione catalase (GSH-Px, A005-1-2, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) kits were used to check the levels of GSH-Px contents in serum according to the instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!