Rabbit anti β actin polyclonal antibody

Human hepatoma Huh7 cells (a generous gift from Dr V. Lotteau, CIRI, Lyon) were cultured in DMEM medium supplemented with 10% heat-inactivated fetal bovine serum (Dutscher, Brumath, France) and antibiotics (PAN Biotech Dutscher, Brumath, France) at 37 °C under a 5% CO₂ atmosphere. The mouse anti-DDDDK tag (FLAG) antibody (Abcam, Cambridge, UK) in PBS, rabbit DENV-2 NS1 polyclonal antibody PA5-33207 (anti-NS1 pAb), and goat Alexa 488-conjugated anti-mouse IgG antibody were purchased from Thermo Fisher Scientific (Les Ulis, France) in PBS. The mouse anti-flavivirus NS1 antibody [D/2/D6/B7] (anti-NS1 mAb) and anti-mouse IgG HRP-conjugated secondary antibodies were purchased from Abcam (Cambridge, UK). The rabbit anti-β actin polyclonal antibody was purchased from ABclonal (MA, Woburn, USA).

Human embryonic kidney HEK-293T cells (ATCC, CRL-1573) were cultured in minimum essential media (MEM) supplemented with 5% heat-inactivated fetal bovine serum (FBS) and human hepatoma Huh7 cells were cultured in DMEM medium supplemented with 10% heat-inactivated fetal bovine serum (FBS, Dutscher, Brumath, France). Both cell lines were cultured with antibiotics (PAN Biotech Dutscher, Brumath, France) at 37 °C under a 5% CO₂ atmosphere. Mouse anti-DDDDK tag monoclonal antibody (FLAG Ab) and mouse anti-6x(His) monoclonal antibody was purchased from Abcam (Cambridge, UK). The mouse anti-flavivirus NS1 antibody [D/2/D6/B7] (NS1 mAb^D/2/D6/B7), which reacts with a conformational epitope present on DENV-2, was purchased from Abcam (Cambridge, UK). The rabbit dengue virus type 2 NS1 polyclonal antibody PA5-33207 (NS1 Ab^PA5-32207) raised against the residues NS1-117/301 was purchased from (Thermo Fisher Scientific, Les Ulis, France). The rabbit anti-β actin polyclonal antibody was purchased from ABclonal (Massachusetts, USA). Goat anti-mouse IgG secondary antibody and Alexa Fluor Plus 488, was purchased from Invitrogen (Thermo Fisher, Les Ulis, France). Anti-mouse and anti-rabbit IgG HRP-conjugated secondary antibodies were purchased from Abcam (Cambridge, UK). DAPI was purchased from Euromedex (Souffelweyersheim, France).