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Cd43 specific beads

Manufactured by Miltenyi Biotec

CD43-specific beads are a laboratory tool used for the isolation and enrichment of CD43-positive cells. CD43, also known as leukosialin, is a sialoglycoprotein expressed on the surface of most leukocytes. These beads are designed to magnetically separate CD43-expressing cells from a complex sample, enabling their further analysis or downstream applications.

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2 protocols using cd43 specific beads

1

Isolation of Naïve, Germinal Center, and Non-Germinal Center B Cells

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Naïve B cells were obtained by negative sorting using CD43-specific beads (Miltenyi) according to manufacturer instructions. Germinal center B cells and non-germinal center B cells were obtained by negative sorting using biotinylated antibodies (CD43, S7,; CD11c, N418; IgD, 11-26 and CD43, S7; CD11c, N418; GL7, respectively. Antibodies obtained from eBioscience), streptavidin-APC (eBioscience) and anti-APC beads (Miltenyi).
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2

Isolation of Naïve, Germinal Center, and Non-Germinal Center B Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Naïve B cells were obtained by negative sorting using CD43-specific beads (Miltenyi) according to manufacturer instructions. Germinal center B cells and non-germinal center B cells were obtained by negative sorting using biotinylated antibodies (CD43, S7,; CD11c, N418; IgD, 11-26 and CD43, S7; CD11c, N418; GL7, respectively. Antibodies obtained from eBioscience), streptavidin-APC (eBioscience) and anti-APC beads (Miltenyi).
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