Eight to twelve-week-old WT and Bves−/− C57BL/6 mice were treated with 4% DSS (Affymetrix, #14489) in the drinking water for 5 days and then sacrificed. Colonoscopy (Karl Storz, Veterinary Endoscopy) was performed before sacrifice and videos were scored using the murine endoscopic index of colitis severity (MEICS),31 which is a 15-point score based on thickening of the colon (0–3), changes of the vascular pattern (0–3), fibrin visibility (0–3), granularity of the mucosal surface (0–3), and stool consistency (0–3). After sacrifice, colons were Swiss-rolled and analyzed for histologic injury in a blinded fashion by a gastrointestinal pathologist (M.K.W.) via calculation of an ulceration score, as previously described.32 Briefly, the scoring system utilized a score of 0–4 to grade the extent of destruction of the crypt architecture. A score of 0 indicated normal crypt architecture, 1 indicated effacement of the bottom third of the crypt, 2 indicated effacement of the bottom 2/3 of the crypt, 3 indicated complete effacement of the crypt, and 4 indicated complete crypt architectural effacement along with an absence of the normal epithelial surface.
Veterinary endoscopy
Manufactured by Storz
The Veterinary Endoscopy product is a medical device designed for visual examination of the interior of an animal's body. It consists of a flexible or rigid tube with a camera and lighting system, allowing veterinarians to inspect and diagnose various conditions within the animal's body.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using veterinary endoscopy
1
Colitis Induction and Evaluation in Mice
2
Colitis Induction and Evaluation in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Eight to twelve-week-old WT and Bves−/− C57BL/6 mice were treated with 4% DSS (Affymetrix, #14489) in the drinking water for 5 days and then sacrificed. Colonoscopy (Karl Storz, Veterinary Endoscopy) was performed before sacrifice and videos were scored using the murine endoscopic index of colitis severity (MEICS),31 which is a 15-point score based on thickening of the colon (0–3), changes of the vascular pattern (0–3), fibrin visibility (0–3), granularity of the mucosal surface (0–3), and stool consistency (0–3). After sacrifice, colons were Swiss-rolled and analyzed for histologic injury in a blinded fashion by a gastrointestinal pathologist (M.K.W.) via calculation of an ulceration score, as previously described.32 Briefly, the scoring system utilized a score of 0–4 to grade the extent of destruction of the crypt architecture. A score of 0 indicated normal crypt architecture, 1 indicated effacement of the bottom third of the crypt, 2 indicated effacement of the bottom 2/3 of the crypt, 3 indicated complete effacement of the crypt, and 4 indicated complete crypt architectural effacement along with an absence of the normal epithelial surface.
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