Chemicals, human IgG, and Protein A from Staphylococcus aureus were purchased for Sigma-Aldrich (St. Louis, MO), and HPLC-grade reagents were purchased from Fisher Scientific™ (Waltham, MA). Lys-C was purchased from FUJIFILM Wako Chemicals (Richmond, VA). HEK293 cell line was purchased from American Type Culture Collection (ATCC, Manassas, VA). Yarra-SEC-4000 column (300 × 7.8 mm, pore size 500 Å, particle size 3 μm) was purchased from Phenomenex® (Torrance, CA). Empore™ Extraction Disks (3M C18) were purchased from VWR (Radnor, PA). iRT Kit was purchased from Biognosys Inc. (Boston, MA).
Hplc grade reagents
Manufactured by Thermo Fisher Scientific
HPLC-grade reagents are high-purity chemicals designed for use in high-performance liquid chromatography (HPLC) analysis. These reagents are manufactured to meet stringent specifications for the detection and separation of compounds in complex mixtures. They are formulated to minimize interference and ensure reliable, consistent, and reproducible HPLC results.
Automatically generated - may contain errors
2 protocols using hplc grade reagents
1
Purification and Characterization of IgG
2
Atractylodin Cytotoxicity Assay
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Atractylodin was provided by Shanghai Run-Biotech Co., Ltd. HPLC-grade reagents were provided by Fisher Scientific. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and other chemicals were provided by Sigma Chemical Co.
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