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Emmpo

Manufactured by Thermo Fisher Scientific
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Sourced in United States

The EMMPO is a laboratory equipment designed for the measurement and analysis of various samples. It utilizes electromagnetic principles to conduct its core function. The detailed specifications and intended applications of this product are not available at this time.

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Lab products found in correlation

Naphthol as d chloroacetate esterase, by Merck Group (2 mentions) Ab102531, by Abcam (1 mentions) Ab253217, by Abcam (1 mentions) Ab253219, by Abcam (1 mentions) Ab102523, by Abcam (1 mentions) Emlcn2, by Thermo Fisher Scientific (1 mentions) Eiasodc, by Thermo Fisher Scientific (1 mentions)

6 protocols using emmpo

1

Quantifying Neutrophil Influx in Sepsis

Cited 1 time
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MPO levels in the spleen, as an assessment of neutrophil influx in tissue homogenates, were measured 24 h post-CLP by ELISA according to the manufacturer’s instructions (#EMMPO; Thermo Fisher Scientific, Waltham, Mass). Similar to cytokine analysis, the 24 h time point was selected as myeloid cells are known to respond robustly and rapidly to septic stimuli within this time frame. To measure esterase levels, spleen sections were stained with naphthol AS-D chloroacetate esterase (Sigma-Aldrich, St. Louis, Mo) according to the manufacturer’s instructions. The images were collected with the Nikon Eclipse 80i microscope using a ×20 and ×40 objective and Spot RT3 camera. Slides were randomly screened and blindly evaluated with three to six images acquired per specimen (42 (link)–44 (link)).
Phares T.W., Kotraiah V., Chung C.S., Unsinger J., Mazer M., Remy K.E., Browne C.D., Buontempo P., Mansour M., Pannucci J., Ayala A., Hotchkiss R.S, & Gutierrez G.M. (2021). A PEPTIDE-BASED CHECKPOINT IMMUNOMODULATOR ALLEVIATES IMMUNE DYSFUNCTION IN MURINE POLYMICROBIAL SEPSIS. Shock (Augusta, Ga.), 55(6), 806-815.
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2

Biomarker Quantification Protocol

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The amylase (#ab102523, Abcam), myeloperoxidase level (#EMMPO, Thermo Fisher Scientific), trypsin (#ab102531, Abcam), 4-HNE (#STA-838, Cell Biolabs), 8-OHG (#K4160, BioVision), IL-6 (#EH2IL6 and #KMC0061, Thermo Fisher Scientific), and NOS2 (#ab253219 and #ab253217, Abcam) concentrations or activity in the samples were measured using ELISA kits.
Dai E., Han L., Liu J., Xie Y., Zeh H.J., Kang R., Bai L, & Tang D. (2020). Ferroptotic damage promotes pancreatic tumorigenesis through a TMEM173/STING-dependent DNA sensor pathway. Nature Communications, 11, 6339.
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3

Quantifying Neutrophil Influx in Sepsis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
MPO levels in the spleen, as an assessment of neutrophil influx in tissue homogenates, were measured 24 h post-CLP by ELISA according to the manufacturer’s instructions (#EMMPO; Thermo Fisher Scientific, Waltham, Mass). Similar to cytokine analysis, the 24 h time point was selected as myeloid cells are known to respond robustly and rapidly to septic stimuli within this time frame. To measure esterase levels, spleen sections were stained with naphthol AS-D chloroacetate esterase (Sigma-Aldrich, St. Louis, Mo) according to the manufacturer’s instructions. The images were collected with the Nikon Eclipse 80i microscope using a ×20 and ×40 objective and Spot RT3 camera. Slides were randomly screened and blindly evaluated with three to six images acquired per specimen (42 (link)–44 (link)).
Phares T.W., Kotraiah V., Chung C.S., Unsinger J., Mazer M., Remy K.E., Browne C.D., Buontempo P., Mansour M., Pannucci J., Ayala A., Hotchkiss R.S, & Gutierrez G.M. (2021). A PEPTIDE-BASED CHECKPOINT IMMUNOMODULATOR ALLEVIATES IMMUNE DYSFUNCTION IN MURINE POLYMICROBIAL SEPSIS. Shock (Augusta, Ga.), 55(6), 806-815.
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4

Neutrophil LCN-2 and MPO Quantification

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Neutrophils were lysed in 100 µL of complete extraction buffer (Abcam, Boston, MA, USA, Cat# ab193970) and the levels of LCN-2 (Invitrogen, USA; Cat# EMLCN2) and MPO (Invitrogen, USA; Cat# EMMPO) were evaluated using the manufacturer’s protocol.
Boyce M., Xin Y., Chowdhury O., Shang P., Liu H., Koontz V., Strizhakova A., Nemani M., Hose S., Zigler JS J.r., Campbell M., Sinha D., Handa J.T., Kaarniranta K., Qian J, & Ghosh S. (2022). Microglia–Neutrophil Interactions Drive Dry AMD-like Pathology in a Mouse Model. Cells, 11(22), 3535.
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5

Suture-Induced Tissue Response in Rats

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SD rats (Male, 230–250 g) were anesthetized and mechanically ventilated by inhalation of isoflurane (RWD, R510-22-10). The DTMS and 5-0 silk sutures were threaded through the rat’s back tissue and surgically knotted. After 7 days, the tissue was fixed and stained with HE, and the tissue homogenate was extracted and used for ELISA detection (Invitrogen, A43656; EMMPO).
Xue F., Zhao S., Tian H., Qin H., Li X., Jian Z., Du J., Li Y., Wang Y., Lin L., Liu C., Shang Y., He L., Xing M, & Zeng W. (2024). Two way workable microchanneled hydrogel suture to diagnose, treat and monitor the infarcted heart. Nature Communications, 15, 864.
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6

Quantification of Inflammatory Markers in Murine Colitis

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The levels of mouse interleukin (IL)-1β (Nanjing Senbeijia Biological Technology, Nanjing, China), IL-6 (Nanjing Senbeijia Biological Technology), tumor necrosis factor alpha (TNF-α) (Nanjing Senbeijia Biological Technology), IL-10 (Nanjing Senbeijia Biological Technology), plateletactivating factor (PAF; cat No. MBS2700185; MyBioSource, San Diego, USA), prostaglandin E2 (PGE2, MyBioSource), myeloperoxidase (MPO; cat. No. EMMPO; Invitrogen, Waltham, USA), and superoxide dismutase (SOD; cat. No. EIASODC; Thermo Fisher Scientific, Waltham, USA) were assessed in the colon homogenates and serum samples, using the abovementioned commercially available kits according to the manufacturer's specifications. 34 (link)
Zhou A., Zhang S., Yang C., Liao N, & Zhang Y. (2022). Dandelion root extracts abolish MAPK pathways to ameliorate experimental mouse ulcerative colitis. Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 31(5).
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