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Mper lysis

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MPER lysis is a buffer solution designed for cell lysis and protein extraction. It is used to solubilize cell membranes and release intracellular proteins for further analysis. The buffer composition facilitates the extraction of membrane-associated and hydrophobic proteins.

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Lab products found in correlation

Ab92513, by Abcam (2 mentions) Ab52615, by Abcam (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Cyclophilin b, by Cell Signaling Technology (2 mentions) Ecl prime, by Cytiva (2 mentions)

2 protocols using mper lysis

1

Detecting Recombinant and Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
For detection of recombinant proteins, 2 μl of Grafix and DSSO fractions were diluted 1:20 and run on 4-12% SDS-PAGE, transferred to PDVF, and detected by ECL Prime (Cytiva Life Sciences) using HRP-anti-rabbit IgG. For detection of protein from mammalian cells, total protein was extracted by MPER lysis (Thermofisher Scientific), quantitated by BCA assay (Pierce), and 10 μg lysate resolved by 4-12% SDS-PAGE. The following antibodies were used: androgen receptor antibody (Abcam ab52615 - 1:1000 for lysates, 1:5000 for recombinant protein), ERG (Abcam ab92513 - 1:1000 for lysates, 1:2000 for recombinant protein), B Actin (Cell Signaling 4970S - 1:5000,) and Cyclophilin B (Cell Signaling 43603S - 1:5000).
Wasmuth E.V., Vanden Broeck A., LaClair J.R., Hoover E.A., Lawrence K.E., Paknejad N., Pappas K., Matthies D., Wang B., Feng W., Watson P.A., Zinder J.C., Karthaus W.R., de la Cruz M.J., Hite R.K., Manova-Todorova K., Yu Z., Weintraub S.T., Klinge S, & Sawyers C.L. (2022). Allosteric interactions prime androgen receptor dimerization and activation. Molecular cell, 82(11), 2021-2031.e5.
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2

Detecting Recombinant and Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
For detection of recombinant proteins, 2 μl of Grafix and DSSO fractions were diluted 1:20 and run on 4-12% SDS-PAGE, transferred to PDVF, and detected by ECL Prime (Cytiva Life Sciences) using HRP-anti-rabbit IgG. For detection of protein from mammalian cells, total protein was extracted by MPER lysis (Thermofisher Scientific), quantitated by BCA assay (Pierce), and 10 μg lysate resolved by 4-12% SDS-PAGE. The following antibodies were used: androgen receptor antibody (Abcam ab52615 - 1:1000 for lysates, 1:5000 for recombinant protein), ERG (Abcam ab92513 - 1:1000 for lysates, 1:2000 for recombinant protein), B Actin (Cell Signaling 4970S - 1:5000,) and Cyclophilin B (Cell Signaling 43603S - 1:5000).
Wasmuth E.V., Vanden Broeck A., LaClair J.R., Hoover E.A., Lawrence K.E., Paknejad N., Pappas K., Matthies D., Wang B., Feng W., Watson P.A., Zinder J.C., Karthaus W.R., de la Cruz M.J., Hite R.K., Manova-Todorova K., Yu Z., Weintraub S.T., Klinge S, & Sawyers C.L. (2022). Allosteric interactions prime androgen receptor dimerization and activation. Molecular cell, 82(11), 2021-2031.e5.
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