Log In Sign up
The largest database of trusted experimental protocols

Human slpi

Manufactured by R&D Systems
Visit Supplier

Human SLPI is a secretory leukocyte protease inhibitor. It is a serine protease inhibitor that inhibits various proteases, including human leukocyte elastase, cathepsin G, and chymotrypsin.

Automatically generated - may contain errors

Lab products found in correlation

Mouse slpi, by R&D Systems (2 mentions) Cat no mca2259, by Bio-Rad (2 mentions) Rat anti mouse ige hrp, by Southern Biotech (2 mentions) Il 17a, by BioLegend (2 mentions)

Spelling variants of this product

Human SLPI Quantikine ELISA Kit (3 citations) Recombinant human (rh)SLPI (3 citations) Human SLPI ELISA Kit (2 citations) Recombinant human SLPI (2 citations)

2 protocols using human slpi

1

Quantification of Serum OVA-Specific IgE

Check if the same lab product or an alternative is used in the 5 most similar protocols
IL-17A (Biolegend) and mouse SLPI (R&D Systems DY1735–05), and human SLPI (R&D Systems DY1274–05) ELISAs were performed according to the manufacturer’s protocol. Mouse serum OVA-specific IgE was quantified by sandwich ELISA (Zuberi et al., 2000 (link)). Briefly, plates were coated with 10μg/mL of OVA overnight at 4°C and then blocked with PBS 1x + 0.1% BSA. Serum (1:25 dilution) was placed in the wells and incubated for 2 h at room temperature. OVA-specific monoclonal IgE (Bio-Rad Cat No. MCA2259. Clone 2C6) was used as the standard curve. The bound IgE was detected by Rat anti-mouse IgE-HRP (Southern Biotech Cat No. 1130–05. Clone 23G3).
Jaeger N., McDonough R.T., Rosen A.L., Hernandez-Leyva A., Wilson N.G., Lint M.A., Russler-Germain E.V., Chai J.N., Bacharier L.B., Hsieh C.S, & Kau A.L. (2020). Airway Microbiota-Host Interactions Regulate Secretory Leukocyte Protease Inhibitor Levels and Influence Allergic Airway Inflammation. Cell reports, 33(5), 108331.
+ Open protocol
+ Expand
2

Quantification of Serum OVA-Specific IgE

Check if the same lab product or an alternative is used in the 5 most similar protocols
IL-17A (Biolegend) and mouse SLPI (R&D Systems DY1735–05), and human SLPI (R&D Systems DY1274–05) ELISAs were performed according to the manufacturer’s protocol. Mouse serum OVA-specific IgE was quantified by sandwich ELISA (Zuberi et al., 2000 (link)). Briefly, plates were coated with 10μg/mL of OVA overnight at 4°C and then blocked with PBS 1x + 0.1% BSA. Serum (1:25 dilution) was placed in the wells and incubated for 2 h at room temperature. OVA-specific monoclonal IgE (Bio-Rad Cat No. MCA2259. Clone 2C6) was used as the standard curve. The bound IgE was detected by Rat anti-mouse IgE-HRP (Southern Biotech Cat No. 1130–05. Clone 23G3).
Jaeger N., McDonough R.T., Rosen A.L., Hernandez-Leyva A., Wilson N.G., Lint M.A., Russler-Germain E.V., Chai J.N., Bacharier L.B., Hsieh C.S, & Kau A.L. (2020). Airway Microbiota-Host Interactions Regulate Secretory Leukocyte Protease Inhibitor Levels and Influence Allergic Airway Inflammation. Cell reports, 33(5), 108331.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!