mice were used according to the guidelines of the Chancellor's Animal Research
Committee of the University of California, Los Angeles. In addition, the ARRIVE (Animal
Research: Reporting In Vivo Experiments) guidelines for the execution and submission of
studies in animals were followed (20 (link)). Mice were
fed a soft diet (Bio Serve®, Frenchtown, NJ) ad libitum for the
duration of the experiment. Eight animals/implants were included in the control group and
10 animals/implants in the experimental group (ligature, peri-implantitis). At the end of
the experimental period, there were eight remaining implants in the control group and six
in the experimental group. No other adverse events were observed in the animals other than
loss of implant osseointegration. All the procedures performed in the mice including tooth
extraction, implant placement and ligature placement were performed under 10×
magnification (Leica S6D Stereozoom, Chicago, IL).