S6d stereozoom

Manufactured by Leica

Sourced in Switzerland

The Leica S6D Stereozoom is a compact and versatile stereomicroscope designed for laboratory use. It features a zoom magnification range, providing a flexible view of specimens. The S6D offers high-quality optics and a stable construction, enabling precise observations and analyses.

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Lab products found in correlation

C57bl 6j, by Jackson ImmunoResearch (1 mentions) Millicell cm culture plate insert, by Merck Group (1 mentions) 24 well culture plate, by Corning (1 mentions) Matrigel, by BD (1 mentions) Millicell cell culture insert, by Merck Group (1 mentions)

4 protocols using s6d stereozoom

Murine Model of Peri-Implant Disease

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Eighteen four-week-old C57BL/6J (The Jackson Laboratories, Bar Harbor, ME) male
mice were used according to the guidelines of the Chancellor's Animal Research
Committee of the University of California, Los Angeles. In addition, the ARRIVE (Animal
Research: Reporting In Vivo Experiments) guidelines for the execution and submission of
studies in animals were followed (20 (link)). Mice were
fed a soft diet (Bio Serve®, Frenchtown, NJ) ad libitum for the
duration of the experiment. Eight animals/implants were included in the control group and
10 animals/implants in the experimental group (ligature, peri-implantitis). At the end of
the experimental period, there were eight remaining implants in the control group and six
in the experimental group. No other adverse events were observed in the animals other than
loss of implant osseointegration. All the procedures performed in the mice including tooth
extraction, implant placement and ligature placement were performed under 10×
magnification (Leica S6D Stereozoom, Chicago, IL).

Pirih F.Q., Hiyari S., Barroso A.D., Jorge A.C., Perussolo J., Atti E., Tetradis S, & Camargo P.M. (2014). Ligature-Induced Peri-Implantitis in Mice. Journal of periodontal research, 50(4), 519-524.

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Explant Culture Regulation by CUL3 Silencing

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Explant culture was performed as described previously (Genbacev et al. 1992) (link). In brief, placental anchoring villi (5-8 w) were identified by a phase contrast microscope (Leica S6 D Stereozoom; Leica, Heerbrugg, Switzerland), dissected, and placed on the Millicell-CM culture plate inserts (EMD Millipore Corp.) pre-coated with phenol red-free and growth factor-reduced Matrigel (Becton Dickinson, Bedford, MA, USA). The inserts were then placed into a 24-well culture plate (Costar, Cambridge, MA, USA). The explants were cultured in serum-free DMEM mixed 1:1 with Ham's F-12 (DMEM/F12; HyClone) medium with 500 nM of scrambled control siRNA or CUL3 siRNA (5 0 -AUAACUUGUACAUG-CAACCAAGGUC-3 0 ). Explants were photographed daily for up to 4 days. The distance from the cell column base to the tip of the outgrowth was measured with SPOT Imaging Software (SPOT Imaging Solutions, Diagnostic Instruments, Inc., Sterling Heights, MI, USA). All the explant experiments were repeated three times and replicated in four separate sets of explants.

Zhang Q., Yu S., Huang X., Tan Y., Zhu C., Wang Y.L., Wang H., Lin H.Y., Fu J, & Wang H. (2015). New insights into the function of Cullin 3 in trophoblast invasion and migration. Reproduction (Cambridge, England), 150(2).

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Floating Villi Culture for Early Placentation

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Floating villi at 6-10 weeks of gestation were dissected under a phase-contrast microscope (Leica S6 D Stereozoom). Small pieces (0.5×0.5 cm) of decidua with no anchored villi from the same individual were also collected and mounted onto transwell inserts (Millicell Cell Culture Inserts, 0.4 µm, 12 mm diameter, PICM01250, Merck Millipore) containing matrigel (5 mg/ml). Counterparts from the same decidua were used for immunofluorescence to examine whether trophoblast cells are present or not. After the decidua was well mounted on the matrigel, three to eight floating villous trees without columns were explanted to the upper surface of the mounted decidua. The co-cultured tissues were fixed with 4% PFA after being cultured for 3 days.

Chang W.L., Liu Y.W., Dang Y.L., Jiang X.X., Xu H., Huang X., Wang Y.L., Wang H., Zhu C., Xue L.Q., Lin H.Y., Meng W, & Wang H. (2018). PLAC8, a new marker for human interstitial extravillous trophoblast cells, promotes their invasion and migration. Development (Cambridge, England), 145(2), dev148932.

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Induction of Experimental Periodontitis in Mice

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A modified mouse model of ligature-induced experimental periodontitis was generated based on method previously described.¹ Six WT mice and 8 TLR9 KO mice were randomly selected for each group. On day 0, the silk thread of size 7-0 (Fisher Scientific) were ligated around both maxillary second molars in each mouse and remained for 2 weeks. The palatal gingiva on the left side was injected with a CpG+CD40L mixture (0.1 μg/ml of CD40L + 40 μM CpG) and that on the right side was injected with vehicle control (PBS). Insulin syringes (Gauge 31, 3/10cc, BD Biosciences) were used for the injection. To perform the injection accurately, the tip of each needle was blunted to ensure that its tip was embedded in the gingiva during the procedure. On days 3, 6, and 9, CD40L+CpG or PBS was injected into the palatal gingiva of maxillary second molars of each mouse. We performed the whole procedures of ligature and injection using an optical microscope (S6D Stereozoom, Leica).

Zhao Q., Hu Y., Deng S., Yu P., Chen B., Wang Z, & Han X. (2018). Cytidine-phosphate-guanosine oligodeoxynucleotides in combination with CD40 ligand decrease periodontal inflammation and alveolar bone loss in a TLR9-independent manner. Journal of Applied Oral Science, 26, e20170451.

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