After the rats were euthanized with carbon dioxide, the knee joints of the right hindlimbs were harvested and immersed in 10% formalin for 72 hours. The specimens were decalcified in 20% ethylenediaminetetraacetic acid solution (pH 7.2). The tibiae were hemisected in the midsagittal plane and each half was embedded in a single block of Paraplast X-TRA medium (Sigma-Aldrich, St Louis, MO, USA). Blocks were trimmed to expose cartilage using a rotary microtome (Reichert-Jung, Vienna, Austria). Ten adjacent sections were collected at intervals of 0, 100, and 200 μm. Two serial 6-μm-thick sections from each interval of 0, 100, and 200 μm were stained with Safranin-O/fast green and the severity of cartilage damage was assessed using OOCHAS score (OA score = grade × stage; range, 0–24).30 (link) Three independent blinded observers scored each section, and the scores for the medial and lateral tibial plateau were averaged within each joint.
Paraplast x tra medium
Manufactured by Merck Group
Sourced in United States
Paraplast X-TRA medium is a paraffin-based embedding medium used in histological sample preparation. It is designed to provide a firm, consistent support for tissue sections during microtome sectioning. The medium's composition and properties ensure optimal cutting characteristics for generating high-quality tissue sections.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using paraplast x tra medium
1
Assessing Cartilage Damage in Rat Knee Joints
2
Histopathological Evaluation of Osteoarthritis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the mice were killed with carbon dioxide, the knee joints of right hindlimbs were harvested and immersed in 10% formalin for 72 hours. The specimens were decalcified in 20% ethylenediaminetetraacetic acid solution (pH 7.2). They were processed in a Tissue-Tek VIP 1000 tissue processor (Miles Laboratories, Elkhart, IN, USA) and embedded in a single block of Paraplast X-TRA medium (Sigma-Aldrich, St Louis, MO, USA). Blocks were trimmed to expose tissue using a rotary microtome (Reichert-Jung, Vienna, Austria), and 6-μm coronal sections were mounted on slides. Safranin O staining was performed, and the severity of cartilage damage was assessed using the Osteoarthritis Research Society International Osteoarthritis Cartilage Histopathology Assessment System (OOCHAS) (OA score = grade × stage; range, 0 to 24) [31 (link)]. Three independent blinded observers (LW, HL and JZ) scored each section, and the scores for the medial and lateral tibial condyles were averaged within each joint.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!