Human osteosarcoma MG63 and normal osteoblast hFOB cell lines were purchased from the Cell Bank Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Dulbecco's modi ed Eagle's medium-F12 (DMEM/F12) was purchased from Hyclone, USA; Fetal Bovine Serum (FBS), Penicillin-streptomycin (100 U/mL) were purchased from Hangzhou Sijiqing Biological Company; RIPA Lysis buffer, BCA Protein Quanti cation Reagent Kit were purchased from Invitrogen, USA; Opti-MEM (Gbico, UK); Anti-Tim-3 monoclonal antibody was obtained from BD Bioscience, USA; Rabbit anti-E-Cadherin, N-cadherin, Vimentin, and GAPDH monoclonal antibodies were purchased from Abcam, USA; Rabbit anti-CD9, CD81, and CD63 monoclonal antibodies were purchased from Santa Cruz, USA; Horseradish peroxidase (HRP) labeled goat anti-rabbit IgG secondary antibody was purchased from Guangzhou Jingcai; PKH67 green uorescent cell linker dye, and PMA were purchased from Sigma Company, USA.
Anti tim 3 monoclonal antibody
Manufactured by BD
Sourced in United Kingdom
The Anti-Tim-3 monoclonal antibody is a laboratory research tool used to detect and study the expression of the Tim-3 protein. Tim-3 is a cell surface receptor involved in the regulation of immune responses. The antibody can be used in various immunoassay techniques to identify and quantify Tim-3 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Ripa lysis buffer, by Thermo Fisher Scientific (2 mentions)
Bca protein quanti cation reagent kit, by Thermo Fisher Scientific (2 mentions)
Pkh67 green uorescent cell linker dye, by Merck Group (2 mentions)
Opti mem, by BD (2 mentions)
Rabbit anti e cadherin, by Abcam (2 mentions)
Phorbol myristate acetate (pma), by Merck Group (2 mentions)
Vimentin, by Abcam (2 mentions)
N cadherin, by Abcam (2 mentions)
2 protocols using anti tim 3 monoclonal antibody
1
In vitro Study of Human Osteosarcoma
2
In vitro Study of Human Osteosarcoma
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human osteosarcoma MG63 and normal osteoblast hFOB cell lines were purchased from the Cell Bank Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Dulbecco's modi ed Eagle's medium-F12 (DMEM/F12) was purchased from Hyclone, USA; Fetal Bovine Serum (FBS), Penicillin-streptomycin (100 U/mL) were purchased from Hangzhou Sijiqing Biological Company; RIPA Lysis buffer, BCA Protein Quanti cation Reagent Kit were purchased from Invitrogen, USA; Opti-MEM (Gbico, UK); Anti-Tim-3 monoclonal antibody was obtained from BD Bioscience, USA; Rabbit anti-E-Cadherin, N-cadherin, Vimentin, and GAPDH monoclonal antibodies were purchased from Abcam, USA; Rabbit anti-CD9, CD81, and CD63 monoclonal antibodies were purchased from Santa Cruz, USA; Horseradish peroxidase (HRP) labeled goat anti-rabbit IgG secondary antibody was purchased from Guangzhou Jingcai; PKH67 green uorescent cell linker dye, and PMA were purchased from Sigma Company, USA.
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