Motorized stage

was performed to determine the cytokine production in BMDM of reporter mice (Table S5, Supporting Information) with or without IFNg stimulation. Harvested cells were treated with various combinations of small‐molecules (0–10 µm, DMSO <0.5%) for 24 h by adding prepared stock solutions to cell culture media. Cells were imaged in a 96‐well plate. Before imaging, cells were stained with Hoechst 33 342 (15 µg mL⁻¹, Thermo Fisher) or SYTO 11 Green Fluorescent Nucleic Acid Stain (2.5 µµ, Thermo Fisher) according to the manufacturer's protocol. Fluorescence microscopy was performed using an IX81 inverted fluorescence microscope (Olympus, Tokyo, Japan) equipped with a motorized stage (Renishaw, Wotton‐under‐Edge, England, UK) and fitted with an ORCA‐Fusion Digital CMOS camera (Hamamatsu Photonics, Hamamatsu, Japan). Using CellSens Dimension 3.1.1 software (Olympus), multiple fields of view were acquired for each sample with a UPlanSApo ×10 (numerical aperture (NA) 0.75, Olympus) or a UPlanSApo ×40 air objective (NA 0.95, Olympus). In addition to brightfield, four fluorescent channels were acquired DAPI (345/455 nm), GFP (489/508 nm), YFP (550/565 nm), CY3 (550/565 nm), and CY5 (625/670 nm) were excited with the appropriate optical filters.