Rabbit anti nlrp3 antibodies

Lung tissues were homogenized to obtain protein samples with RIPA lysis buffer containing PMSF after washing with cold PBS. Then, the protein (25 μg) was separated by SDS-PAGE and transferred to PVDF membranes. The membranes were incubated overnight with appropriated primary antibody at 4°C after being blocked with 5% bovine serum albumin (BSA). Rabbit anti-ICAM-1 antibodies (1: 1000), rabbit anti-CXCR4 antibodies (1: 1000), rabbit anti-SDF-1 antibodies (1: 1000), rabbit anti-Bcl2 antibodies (1: 1000), rabbit anti-bax antibodies (1: 1000), rabbit anti-cleaved caspase3 antibodies (1: 500), rabbit anti-NLRP3 antibodies (1: 1000), rabbit anti-ASC antibodies (1: 1000), rabbit anti-pro-IL-1β antibodies (1: 1000), rabbit anti-IL-1β antibodies (1: 1000), rabbit anti-pro-caspase1 antibodies (1: 1000), and rabbit anti-caspase1 antibodies (1: 1000) were purchased from Cell Signaling Technology (Danvers, USA). Subsequently, PVDF membranes were incubated with the corresponding secondary antibodies and visualized using a Tanon-5200 Chemiluminescence Imager (Tanon, Shanghai, China) with enhanced chemiluminescence (ECL) method.