Cell proteomes were separated by SDS-PAGE, transferred to nitrocellulose membrane (60 V for 90 min), and blocked by 5% milk in TBS-Tween. The primary antibodies used and dilutions are as follows: anti-AIG1 (Rabbit, Sigma-Aldrich, SAB1304597, 1:250), anti-ADTRP (Rabbit, Atlas, HPA048113, 1:500), and anti-β-actin (Mouse, Cell Signaling, 3700S, 1:1000). IRDye 800CW anti-rabbit and anti-mouse secondary antibodies (LI-COR, 1:10000) were used as secondary antibodies for visualization.
Anti aig1
Manufactured by Merck Group
Anti-AIG1 is a laboratory equipment product manufactured by Merck Group. It is used for the detection and quantification of AIG1, a specific protein target. The core function of Anti-AIG1 is to facilitate the analysis and measurement of AIG1 levels in various samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti aig1
1
Western Blot Analysis of Protein Expression
2
Western Blot Analysis of Protein Expression
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