Thermo biomate 5

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Thermo BioMate 5 is a UV-Vis spectrophotometer designed for use in life science laboratories. It measures the absorbance of light in the ultraviolet and visible wavelength ranges, allowing for the quantification of various biomolecules such as proteins, nucleic acids, and other compounds. The core function of the Thermo BioMate 5 is to provide accurate and reliable absorbance measurements for analytical and research applications.

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Thermo Biomate 5 Spectrophotometer Biomate 5

2 protocols using thermo biomate 5

Optimizing Surface-Enhanced Raman Scattering Colloids

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Multiple batches of these three colloids were assessed by UV-visible spectroscopy (Thermo BioMate 5; Thermo Fisher Scientific, Inc., MA, USA), and spectra were very similar to those we had synthesised previously [15 (link), 17 (link), 24 (link)]. Initial SERS optimisation established that the silver borohydride colloid and gold citrate colloid did not produce very reproducible SERS data and thus were abandoned in this investigation.
Electron microscopy of multiple batches of the silver citrate colloid using a Zeiss Supra 40 VP field-emission gun scanning electron microscope (FEG-SEM; Carl Zeiss SMT GmBH, Oberkochen, Germany) operating at a voltage of 1 kV established that we had produced nanoparticles of 70 ± 30 nm (data not shown).

Alharbi O., Xu Y, & Goodacre R. (2015). Simultaneous multiplexed quantification of caffeine and its major metabolites theobromine and paraxanthine using surface-enhanced Raman scattering. Analytical and Bioanalytical Chemistry, 407(27), 8253-8261.

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Determining Plasmonic Nanoparticle Properties

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In order to determine the position of the plasmon band λ max it was essential to characterise the nanoparticles using UV-vis spectrophotometry. Samples were prepared by combining 1 part silver colloid with 9 parts water. 1 mL of the dilute nanoparticle solution was then pipetted into a quartz cuvette and inserted into a sample holder of a Thermo Biomate 5 (Thermo Fisher Scientific Inc., Massachusetts, USA). A spectrum was collected for each of the 5 colloidal batches. Fig. 2 shows the typical UV-Vis spectra obtained whilst the table details the λ max and full width, half maximum (FWHM) for each batch of colloid.

Mabbott S., Alharbi O., Groves K, & Goodacre R. (2015). Application of surface enhanced Raman scattering to the solution based detection of a popular legal high, 5,6-methylenedioxy-2-aminoindane (MDAI). The Analyst, 140(13).

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