Trypsin edta

Bletilla striata (BS) was purchased from Sheng Chang Corporation (Taoyuan, Taiwan), and the WST-1 cell proliferation assay kit was obtained from Takara Bio USA, Inc. (Mountain View, CA, USA). Antibiotics, trypsin–EDTA, DMEM, MEM and FBS were purchased from GE Healthcare (Little Chalfont, UK). Live/dead kit, SuperScript first-strand synthesis system for RT-PCR, high-capacity cDNA reverse transcription kits, TaqMan real-time PCR master mixes, probes, and TRIzol were purchased from ThermoFisher Scientific (Waltham, MA, USA). All other chemicals were purchased from Sigma-Aldrich (St Louis, MO, USA).

Glutamine, DMEM, trypsin-EDTA, and penicillin/streptomycin were obtained from GE Healthcare HyClone (Logan, UT, United States). Fetal bovine serum, enzymes, 3-aminopropyltriethoxy silane (TESPA), L-15 and chemicals were from Sigma (St. Louis, MO, United States). DiI-C₁₂, DiD-C₁₈, Lipofectamine 2000 and Gibco Opti-MEM were from Thermo Fisher Scientific (Waltham, MA, United States). High performance, 1.5H, coverslips were from Marienfeld (Lauda-Königshofen, Germany). The colon cancer cell line HT29 was a kind gift of Dr. A. Blokzijl, Uppsala University, Sweden and SW480 cells were from ATCC (Manassas, VA, United States). CD3ζ-EYFP, mouse CD3ζ fused to EYFP via a six aa linker expressed in the pBJ1-Neo plasmid under the CMV-promotor, was from Mark Davis, Stanford University School of Medicine, United States (Krummel et al., 2000 (link)). Lck-EGFP, a fusion protein of mouse Lck and EGFP via a six aa linker expressed in the pcDNA3 under the CMV-promotor, was from Tony Magee, Imperial College London, United Kingdom (Janes et al., 1999 (link)). Borosilicate glass (1B100F-4) was from World Precision instruments (Sarasota, FL, United States).

Chemical reagents were obtained from Sigma–Aldrich (St. Louis, MO, USA) unless otherwise specified. RPMI1640, fetal bovine serum (FBS), and penicillin/streptomycin were purchased from Invitrogen Life Technologies (Carlsbad, CA, USA). Trypsin/EDTA was purchased from GE Healthcare Life Sciences (Logan, UT, USA). E-Cadherin rabbit polyclonal antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). AMPK-α rabbit polyclonal antibody, rabbit monoclonal antibodies specific to p-AMPK-α (Thr172), JAK2, p-JAK2 (Tyr1008), and occludin, and mouse monoclonal antibodies detecting STAT3, p-STAT3 (Tyr705), cleaved caspase 1, and IL-1β were purchased from Cell Signaling Technology Inc. (Boston, MA, USA). NOX2 and ICAM-1 rabbit monoclonal antibodies, and TGF-β, TNF-α, NOX1, p-p47phox, pro-caspase-1, and claudin 2 rabbit polyclonal antibodies were purchased from Abcam (Cambridge, MA, USA). Rabbit polyclonal antibodies specific for IL-10 or IL-6 were from Abbiotec (San Diego, CA, USA), and NLRP3 rabbit polyclonal antibody was purchased from Novus Biologicals (Centennial, CO, USA). Tofacitinib citrate salt was obtained from L.C Laboratories (Woburn, MA, USA). VAS2870 was purchased from Sigma-Aldrich (St. Louis, MO, USA). BJ-3105 was synthesized by Byeong-Seon Jeong as reported [47 ].

DMSO (Sigma-Aldrich, St. Louis, MO, USA); 0.05% Trypsin-EDTA (GE Healthcare Life Sciences, Little Chalfont, UK); 1% BSA (Serva, Germany) in PBS with 0.01% NaN₃ (Sigma-Aldrich); 0.25% Triton X-100 (Sigma-Aldrich) solution in PBS; 4% Paraformaldehyde solution (Sigma-Aldrich) in PBS; Aldehyde dehydrogenase-based cell detection kit (StemCell 01700); Fumitremorgin C (Abcam, ab144258); For detail specification regarding used antibodies - see Supplementary Tables S1–S3).