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RPMI 1640 and DMEM culture media, fetal bovine serum, glutamine, and a streptomycin/penicillin antibiotic solution were all purchased from Invitrogen (Paisley, UK). Bacterial LPS (Escherisha coli 026:B6), ZnPyr (zinc salt), TTM, TPEN, DTPA, pyrithione, CA, glyburide, and bafilomycin were purchased from Sigma (Gillingham, UK). Ac-YVAD-CHO, IETD-CHO, and Ca-074-Me were purchased from Merck Chemicals Ltd (Darmstadt, Germany). Salicylaldehyde isonicotinoyl hydrazone (SIH) was purchased from ChemBridge (San Diego, CA, USA). FluoZin-3 and Lysotracker Red were purchased from Invitrogen. The anti-mouse IL-1β antibody was from R&D Systems (Abingdon, UK). The XIAP and caspase-8 antibodies were purchased from Cell Signalling (Danvers, MA, USA), the actin antibody was from Sigma, and the caspase-1 antibody was from Santa Cruz Biotechnology (Dallas, TX, USA). Aβ peptide (1-42) was purchased from rPeptide (Bogart, GA, USA).

RPMI 1640 medium and DMEM, fetal bovine serum (FBS), glutamine, and a streptomycin/penicillin antibiotic solution were all purchased from Invitrogen. Bacterial lipopolysaccharide (LPS, Escherichia coli 026:B6) and staurosporine (STS)² were purchased from Sigma. Ac-YVAD-CHO, IETD-CHO, cycloheximide (CHX), ALLN, calpain inhibitor III, EST, and PD150606 were purchased from Merck Chemicals, Ltd. CHX causes apoptosis by inhibiting protein translation and subsequently cell growth, whereas STS is a broad spectrum kinase inhibitor that induces apoptosis. Z-VAD-fmk was purchased from Promega. Necrostatin-1 was purchased from Sigma. The anti-mouse IL-1β and IL-1α antibodies were from R&D Systems. Secondary antibody HRP conjugates were from DAKO.