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Yeast nitrogen base

Manufactured by Lab M
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Sourced in United Kingdom

Yeast nitrogen base is a growth medium used for cultivating and maintaining yeast cultures. It provides essential nutrients, including nitrogen sources, vitamins, and mineral salts, required for yeast proliferation and metabolism.

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Lab products found in correlation

Yeast extract, by Lab M (3 mentions) Peptone, by Lab M (2 mentions) Trametes versicolor, by Merck Group (1 mentions) 1 4 benzoquinone bq, by Merck Group (1 mentions) Melibiose, by Merck Group (1 mentions) D galactose, by Merck Group (1 mentions) Lactose, by Merck Group (1 mentions) Raffinose, by Merck Group (1 mentions) Locust bean galactomannan, by Merck Group (1 mentions) Guar galactomannan, by Merck Group (1 mentions)

3 protocols using yeast nitrogen base

1

Enzymatic Hydrolysis of Xylooligosaccharides

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Below mentioned growth medium chemicals, yeast extract, yeast nitrogen base, and peptone were purchased from Lab M Ltd. (UK). Salts and vitamins were obtained from Sigma-Aldrich or Merck (Germany). Neutral substrates X2, X3, X4, 32-α-L-arabinofuranosyl-xylobiose (A3X), 23-α-L-arabinofuranosyl-xylotriose (A2XX), 33-α-L-arabinofuranosyl-xylotetraose (XA3XX) were purchased from Megazyme (UK). 23-(4-O-methyl-α-D-glucuronyl)-xylotriose (U4m2XX) and 23-(4-O-methyl-α-D-glucuronyl)-xylotetraose (XU4m2XX) substrates were prepared as in Koutaniemi et al. (2012 (link)) by Dr. T. Vuong, University of Toronto and Dr. S. Koutaniemi, University of Helsinki and kindly provided to the study. The commercial laccase from Trametes versicolor (Sigma-Aldrich, Germany) was used in oxidation reactions (described below) to recycle 1,4-benzoquinone (BQ; Sigma-Aldrich, Germany) electron acceptor.
Karppi J., Zhao H., Chong S.L., Koistinen A.E., Tenkanen M, & Master E. (2020). Quantitative Comparison of Pyranose Dehydrogenase Action on Diverse Xylooligosaccharides. Frontiers in Chemistry, 8, 11.
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2

Enzymatic Hydrolysis of Xyloglucan

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Yeast extract, yeast nitrogen base, and peptone were purchased from Lab M Ltd. (UK). d‐galactose, lactose, melibiose, and raffinose were of analytical grade and purchased from Sigma‐Aldrich. Xyloglucan oligosaccharides (hepta‐+octa‐+nonasaccharides) were purchased from Megazyme (O‐XGHON; Lot number 20509). 1,2,3,4‐Di‐O‐isopropylidene‐α‐d‐galactose, used for preparing the synthetic 6‐amino‐6‐deoxy‐d‐galactose used as a standard for product quantification, was purchased from Alfa Aesar. All other chemicals were of reagent grade, obtained from Sigma‐Aldrich (Germany), and used without further purification unless otherwise specified.
Aumala V., Mollerup F., Jurak E., Blume F., Karppi J., Koistinen A.E., Schuiten E., Voß M., Bornscheuer U., Deska J, & Master E.R. (2019). Biocatalytic Production of Amino Carbohydrates through Oxidoreductase and Transaminase Cascades. Chemsuschem, 12(4), 848-857.
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3

Polysaccharide Characterization Protocol

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Yeast extract, yeast nitrogen base and peptone were purchased from Lab M Limited (UK), whereas all other chemicals were reagent grade and purchased from Sigma Aldrich unless otherwise specified. Locust bean galactomannan was purchased from Fluka, whereas galactoxyloglucan from tamarind seed was obtained from Megazyme, and guar galactomannan was obtained from Sigma Aldrich (batch 14K0168). Spruce galactoglucomannan was generously provided by Professor Stefan Wilför (Åbo Academy, Finland), and Pichia pastoris KM71H expressing wild-type GaO was kindly supplied by Professor H. Brumer (University of British Columbia) [26] (link). Reported molecular weights of polysaccharides used in this analysis are as follows: 29 kDa for spruce galactose glucomannan [27] (link), 470 kDa for tamarind galactoxyloglucan, 2600 kDa for guar galactomannan [7] (link), and 310 kDa for Locust bean galactomannan (as stated by Sigma-Aldrich (G0753)).
Mollerup F., Parikka K., Vuong T.V., Tenkanen M, & Master E. (2016). Influence of a family 29 carbohydrate binding module on the activity of galactose oxidase from Fusarium graminearum. Biochimica et biophysica acta, 1860(2).
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