Fluorescence measurements were recorded using a Perkin Elmer LS55 fluorimeter, UV-visible spectrophotometry was performed on a V-660 spectrometer (Jasco (UK) Ltd). Vesicles were extruded using a LiposoFast liposome extruder (AVESTIN Europe GmbH). Sonication was performed using a VCX130PB probe-type sonicator (Sonics & Materials Inc., 130 W power rating). Centrifugation was performed using a Heraeus Instruments Megafuge 1.0R for large tubes and a Beckman Coulter Microfuge 16 for Eppendorf tubes. Vortex mixing was performed using a Vortex Genie 2 (600-2700 rpm). Transmission electron microscopy (TEM) was performed using a Jeol 1220, 120 kV instrument with a GATAN ORIUS CCD camera or a Tecnai T20, 220 kV. Inductive heating of nanoparticles was conducted using a water-cooled EASYHEAT 0224 induction heater (2.0 kW, 150-400 kHz) with an EASYHEAT 300P workhead (392 kHz, 3 cm loop diameter, 1.5 turns). QCM-D was performed using a Q-Sense E4 instrument (Biolin Scientific, UK). NdFeB magnets ring/cylinder shape (5600 Gauss) were obtained from Magnet Expert Ltd (UK).
Liposofast liposome extruder
Manufactured by Avestin
The LiposoFast liposome extruder is a laboratory instrument designed to produce uniform liposomes. It uses a mechanical process to extrude lipid solutions through polycarbonate membranes, creating liposomes of a defined and reproducible size.
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Lab products found in correlation
2 protocols using liposofast liposome extruder
1
Biophysical Characterization of Nanoparticles
2
Preparing Carboxyfluorescein-Loaded Liposomes
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CF-loaded LUVs were prepared from a lipid mixture of DOPC and DOPE in a molar ratio 6:4 dissolved in chloroform. First, 100 µL of the lipid solution were dried under high vacuum overnight to get a dry lipid thin film. 5(6)-CF was encapsulated within LUVs at sufficient concentration to be self-quenched by rehydration of the lipid film with 500 µL of 50 mM TrisHCl, 120 mM CF at pH 8.5 to form a suspension of polydisperse multilamellar liposomes. This liposome suspension was then subjected to 5 freeze-thaw cycles and extruded 11 times through a 400 nm pore size polycarbonate membrane (Whatman International Ltd) using a LiposoFast liposome extruder (Avestin Inc). The final suspension of monodisperse LUVs was passed through a Sephadex G-25 column to remove the unencapsulated CF via SEC.
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