Temozolomide was obtained from Life Sciences (ALX-420-044; Farmingdale, NY). It was resuspended at 20 mg/mL in 100% DMSO, aliquoted and stored at − 20 °C at a concentration of 100 mM. The DMSO concentration in the TMZ, CAP, and combined CAP–TMZ treatment variables was maintained at 0.25% for all experiments. The human cancer cell line, U87MG (glioblastoma) was purchased from the American Type Culture Collection (ATCC) (Manassas, VA). Glioblastoma cells were passaged in cell culture in Dulbecco’s Modified Eagle Medium (DMEM) (Life Technologies) supplemented with 5% (v/v) fetal bovine serum (ThermoFisher) and 1% (v/v) Penicillin–Streptomycin (Life Technologies). Cells were harvested after three passages and were seeded in 24 well plates for performing experiments. The cell culture media utilized for all experiments was prepared with 1% (v/v) fetal bovine serum and 1% (v/v) Penicillin–Streptomycin.
U87mg glioblastoma
Manufactured by American Type Culture Collection
U87MG is a cell line derived from a human glioblastoma, a type of brain cancer. It is a widely used model for the study of glioblastoma biology and experimental therapeutics.
Automatically generated - may contain errors
2 protocols using u87mg glioblastoma
1
Temozolomide and Chloroquine Phosphate Combined Treatment in Glioblastoma
2
Orthotopic Glioblastoma Mouse Model
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Example 8
For the growth of orthotopic glioblastoma, male Balb/c-nu mice (Japan SLC) free of a specific pathogen were used. Intracerebral transplantation of U-87MG glioblastoma (2×105 cells, American Type Culture Collection) was performed according to known methods. The day after the intracerebral transplantation of the glioblastoma, the mice were divided into two groups (n=4), and treated, as follows: (a) intraperitoneal injection of PBS, and (b) intraperitoneal injection of 0.5 mg/kg of DIG-0001. All the treatments were performed 5 times (Days 15, 18, 21, 24 and 27 after inoculation of neoplastic cells). Day 29 after the transplantation of the neoplastic cells, the mice were sacrificed, their brains were removed, and coronally sectioned. One fragment was fixed with 10% buffered formalin, and embedded into formalin, and the remainders were embedded into an OCT compound. A tumor volume was obtained by measuring a volume of a fragment having the highest tumor region, and calculated according to the following equation: Width2×Length×0.5 (
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