T cells were purified from pooled lymph nodes and spleens of wild-type and LckS59A mice with Dynabeads Untouched Mouse T cell kits (Life Technologies) with a cell purity of over 90%. Purified cells were stimulated for 72 h with plate-bound anti-CD3 (145-2C11; American Type Culture Collection) with or without soluble anti-CD28 (37–51; American Type Culture Collection). Intracellular IL-2 and IFN-γ were measured as described (Mingueneau et al, 2008 (link)). Proliferation was measured using CFSE labelling. After 3 days, the absolute numbers of T cells having diluted CFSE were counted after adding Flow-Count™ Fluorospheres (Beckman Coulter).
Anti cd3 145 2c11
Manufactured by American Type Culture Collection
Anti-CD3 (145-2C11) is a monoclonal antibody that binds to the CD3 complex on the surface of T cells. The CD3 complex is involved in signal transduction and T cell activation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti cd3 145 2c11
1
T Cell Activation and Proliferation
2
Expansion and Stimulation of Purified CD4+ T Cells
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