All 45 collected oral cavity swabs from the participants were swabbed directly onto Blood Agar (5% sheep blood), and MacConkey agar for the primary isolation of microaerophilic and aerobic microflora was incubated at 37 °C for 24 hrs in a microaerophilic environment and aerobic environment respectively. mannitol salt agar was used as a selective culture media to isolate S. aureus. In total, 84 colonies having different morphology were isolated and purified after processing the samples. Gram staining was done to identify the suspected isolates of S. aureus. After Gram staining, suspected S. aureus colonies were inoculated on mannitol salt agar (Lab M Limited, UK) and incubated for 24 hrs at 37 °C. Based on physical appearance, golden yellow colored, convex, round, and opaque were the indication of S. aureus strains that were observed. All isolated colonies were shifted to Nutrient agar (Lab M Limited, UK) and incubated at 37 °C for 24 hrs. Once incubated colony characteristics became evident, species were identified depending on the following tests using rapid biochemical tests: oxidase, catalase, coagulase, DNase, and others, according to Bergey’s manual of determinative bacteriology (Vos et al., 2009 ).
Mannitol salt agar
Manufactured by Lab M
Sourced in United Kingdom
Mannitol salt agar is a selective and differential culture medium used for the isolation and identification of Staphylococcus species. It contains a high concentration of sodium chloride, which inhibits the growth of most other bacteria, and mannitol, a carbohydrate that Staphylococcus species can ferment.
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Lab products found in correlation
2 protocols using mannitol salt agar
1
Oral Cavity Microbiome Profiling
2
Enumerating Gut Microbiome of Chicks
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One gram of ileocecal contents of five slaughtered chicks from each group were collected aseptically at the end of the experiment, transferred into a sterile test tube containing 9 ml sterile nutrient broth and diluted by tenfold serial dilution (10-1 to 10-5) then 10 µl [37 ] of mixed diluted sample using vortex (WiseMix, VM-10, Korea) was plated using drop plate technique described by Herigstad et al. [38 (link)] for enumeration of total bacterial count (TBC), total coliform, total streptococci, total Lactobacilli, and total Staphylococci onto the following media: Standard plate count agar (Difco, USA), EMB agar (Lab M, LAB061, UK), Selective Streptococci agar (Biolife, Italia), De Man-Rogosa-Sharpe agar (MRS, Lab M, LAB093, UK), and Mannitol salt agar (Lab M, UK). All plates were cultivated at 37°C for 24-48 h while plates for Lactobacilli were incubated in 3% CO2 atmosphere at 37°C for 48-72 h. The results were expressed as arithmetical means±SE (in log cfu/g).
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