Un scan it gel version

Manufactured by Silk Scientific

The Un-Scan-It gel version is a software tool designed for the digitization and analysis of gel electrophoresis images. It provides functionality for capturing, processing, and quantifying band intensities from gel images.

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Lab products found in correlation

Bca protein assay, by Thermo Fisher Scientific (4 mentions) Novex gels, by Thermo Fisher Scientific (2 mentions) Py1175 vegfr2, by Thermo Fisher Scientific (2 mentions) Flotilin, by Merck Group (2 mentions) Neuropilin 1, by Santa Cruz Biotechnology (2 mentions) Vegfr2, by Thermo Fisher Scientific (2 mentions) Wbkls0500, by Merck Group (2 mentions) Horseradish peroxidase conjugated secondary antibody, by Jackson ImmunoResearch (2 mentions) Rabbit anti β actin, by Abcam (1 mentions) Irdye 800cw goat anti rabbit antibody, by LI COR (1 mentions) Irdye 800cw goat anti mouse antibody, by LI COR (1 mentions) Irdye 680cw goat anti mouse antibody, by LI COR (1 mentions) Bca protein assay kit, by Cole-Parmer (1 mentions) Hybond p pvdf transfer membrane, by Merck Group (1 mentions) Mouse anti glutamine synthetase, by Abcam (1 mentions) Mouse anti gapdh, by Abcam (1 mentions) Odyssey infrared imaging system, by LI COR (1 mentions)

Close spelling variants of this product

UN-SCAN-IT gels (Version 6.1) UN-SCAN-IT

3 protocols using un scan it gel version

Quantification of Hippocampal Proteins

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Protein concentration in hippocampal extracts from mice and human tissue was
measured using the BCA Protein Assay Kit (Cole-Parmer). Forty micrograms of
protein/lane were electrophoretically separated on a 12% SDS polyacrylamide gel
and electrically transferred onto a Hybond-P PVDF transfer membrane (Millipore)
for 1 h. Membranes were blocked in PBS-milk 5% at RT for 1 h. Next, membranes
were incubated in block solution overnight with the following primary
antibodies: mouse anti-glutamine synthetase (1:500; Abcam), mouse anti-GAPDH
(1:1,000; Abcam), rabbit anti-Cyclophilin B (1:1,000; Sigma) and rabbit
anti-β-actin (1:1,000; Abcam). Membranes were incubated for 1 h with IRDye 680CW
goat anti-mouse antibody, IRDye 800CW goat anti-mouse antibody, or IRDye 800CW
goat anti-rabbit antibody (LI-COR, 1:20,000), then scanned with an Odyssey
infrared imaging system (LI-COR) and analyzed using Un-Scan-It gel version 6.1
(Silk Scientific).

Matias I., Diniz L.P., Araujo A.P., Damico I.V., de Moura P., Cabral-Miranda F., Diniz F., Parmeggiani B., de Mello Coelho V., Leite R.E., Suemoto C.K., Ferreira G.C., Kubrusly R.C, & Gomes F.C. (2023). Age-Associated Upregulation of Glutamate Transporters and Glutamine Synthetase in Senescent Astrocytes In Vitro and in the Mouse and Human Hippocampus. ASN NEURO, 15, 17590914231157974.

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Quantitative Western Blot Analysis

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Protein concentrations were determined using the BCA protein assay (Cat# PI23225, Thermo Fisher Scientific, Waltham, MA). Indicated samples were loaded on 4-20% Novex® gels (Cat# EC60285BOX, Thermo Fisher Scientific, Waltham, MA). Proteins were transferred for 1h at 120 V using TGS (25mM Tris pH=8.5, 192mM glycine, 0.1% (mass/vol) SDS), 20% (vol/vol) methanol as transfer buffer to polyvinylidene difluoride (PVDF) membranes 0.45μm (Cat# IPVH00010, Millipore, Billerica, MA), pre-activated in pure methanol. After transfer, the membranes were blocked at room temperature for 1 hour with TBST (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.1 % Tween 20), 5% (mass/vol) non-fat dry milk, then incubated separately in the primary antibodies VEGFR2 (Cat#PA5-16487, ThermoFisher), pY1175 VEGFR2 (Cat#PA5-105167, ThermoFisher), Flotilin (Cat#F1180, Sigma) or Neuropilin-1 (Cat#sc-5307, Santa Cruz biotechnology) in TBST, 5% (mass/vol) BSA, overnight at 4°C. Following incubation in horseradish peroxidase-conjugated secondary antibodies from Jackson immunoresearch, blots were revealed by enhanced luminescence (WBKLS0500, Millipore, Billerica, MA) before exposure to photographic film. Films were scanned, digitized, and quantified using Un-Scan-It gel version 7.1 scanning software by Silk Scientific Inc.

Moutal A., Martin L.F., Boinon L., Gomez K., Ran D., Zhou Y., Stratton H.J., Cai S., Luo S., Gonzalez K.B., Perez-Miller S., Patwardhan A., Ibrahim M.M, & Khanna R. (2020). SARS-CoV-2 Spike protein co-opts VEGF-A/Neuropilin-1 receptor signaling to induce analgesia. bioRxiv.

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Quantification of VEGFR2 Signaling Cascade

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Protein concentrations were determined using the BCA protein assay (Cat# PI23225, Thermo Fisher Scientific, Waltham, MA). Indicated samples were loaded on 4–20% Novex® gels (Cat# EC60285BOX, Thermo Fisher Scientific, Waltham, MA). Proteins were transferred for 1h at 120 V using TGS (25mM Tris pH=8.5, 192mM glycine, 0.1% (mass/vol) SDS), 20% (vol/vol) methanol as transfer buffer to polyvinylidene difluoride (PVDF) membranes 0.45μm (Cat# IPVH00010, Millipore, Billerica, MA), pre-activated in pure methanol. After transfer, the membranes were blocked at room temperature for 1 hour with TBST (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.1 % Tween 20), 5% (mass/vol) non-fat dry milk, then incubated separately in the primary antibodies VEGFR2 (Cat#PA5–16487, ThermoFisher), pY1175 VEGFR2 (Cat#PA5–105167, ThermoFisher), Flotilin (Cat#F1180, Sigma) or Neuropilin-1 (Cat#sc-5307, Santa Cruz biotechnology) in TBST, 5% (mass/vol) BSA, overnight at 4°C. Following incubation in horseradish peroxidase-conjugated secondary antibodies from Jackson immunoresearch, blots were revealed by enhanced luminescence (WBKLS0500, Millipore, Billerica, MA) before exposure to photographic film. Films were scanned, digitized, and quantified using Un-Scan-It gel version 7.1 scanning software by Silk Scientific Inc.

Moutal A., Martin L.F., Boinon L., Gomez K., Ran D., Zhou Y., Stratton H.J., Cai S., Luo S., Gonzalez K.B., Perez-Miller S., Patwardhan A., Ibrahim M.M, & Khanna R. (2020). SARS-CoV-2 spike protein co-opts VEGF-A/neuropilin-1 receptor signaling to induce analgesia. Pain, 162(1), 243-252.

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