Rat monoclonal anti-F4/80 (ab6640), rabbit polyclonal anti-CD45 (ab10558), rabbit monoclonal anti-CD4 (ab183685), rabbit polyclonal anti-CD8 (ab203035), and rat monoclonal anti-B220 (ab64100) antibodies and pan-cytokeratin (ab7753) were purchased from Abcam (Cambridge, UK). Horseradish peroxidase (HRP)-conjugated secondary antibodies and 3,3'-diaminobenzidine (DAB) were purchased from Zhongshan Biotechnology Co. (Beijing, China). Collagenase type IV (C0130-100MG), hyaluronidase (H3506-100MG), and busulfan (S85543-229) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
3 3 diaminobenzidine (dab)
Manufactured by Zhongshan Biotechnology
Sourced in China
3,3'-diaminobenzidine (DAB) is a chromogenic substrate used in immunohistochemistry and enzyme-linked immunosorbent assays (ELISA) to detect the presence of specific target proteins or antigens. It produces a brown-colored precipitate at the site of the enzyme-catalyzed reaction, allowing for visual identification and localization of the target molecule.
Automatically generated - may contain errors
Lab products found in correlation
Ab203035, by Abcam (1 mentions)
Ab64100, by Abcam (1 mentions)
Ab7753, by Abcam (1 mentions)
Ab10558, by Abcam (1 mentions)
Ab183685, by Abcam (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Zhongshan Biotechnology (1 mentions)
Polyethylene glycol, by Merck Group (1 mentions)
Rpmi 1640 culture medium, by Thermo Fisher Scientific (1 mentions)
Sds page gel preparation kit, by Beyotime (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Tween 80, by Merck Group (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
2 protocols using 3 3 diaminobenzidine (dab)
1
Immunohistochemical Analysis of Immune Cells
2
Cell Culture Protocols and Reagents
Check if the same lab product or an alternative is used in the 5 most similar protocols
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RPMI-1640 culture medium and fetal bovine serum (FBS) for cell culture were purchased from Gibco BRL, Gaithersburg, MD, USA. Rabbit anti-Cox-2 monoclonal antibody, mouse anti-PKC-α and mouse anti-P-gp monoclonal antibodies were purchased from Santa Cruz Corporation, CA, USA. Secondary antibodies against the rabbit and mouse antibodies, streptavidin-peroxidase (SP) kits, and 3,3’-diaminobenzidine (DAB) were obtained from Beijing Zhongshan Biotechnology, China. BCA protein assay kits and SDS-PAGE gel preparation kits were obtained from the Beyotime Institute of Biotechnology, Shanghai, China. PAB was purchased from the Liaoning Institute for Drug Control, China No. 201003 (the purity:>98%). Adriamycin (ADR) was obtained from Zhejiang Hisun Pharmaceutical Co., Ltd., China No. 120906. Tween80 and polyethylene glycol were purchased from Sigma Chemical, St. Louis, MO, USA.
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