Anti cyclin a

Protein preparation and Western blots were performed as described previously15 . Nuclear and cytoplasmic lysates were prepared with the NE-PER kit (Pierce Biotechnology) according to the manufacturer’s instructions. The protein levels of active β-catenin in whole-cell lysates were quantified using the anti-active β-catenin clone 8E7 antibody (Millipore). The antibody is specific for the active form of β-catenin, dephosphorylated on Ser37 or Thr41. The following primary antibodies were used: anti-menin (1:1,000; Cell Signalling Technology), anti-β-catenin (1:1,000; Cell Signalling Technology), anti-active β-catenin (1:1,000; Millipore), anti-Phospho-β-catenin (Ser33/37/Thr41; 1:1,000; Cell Signalling Technology), anti-Phospho-β-catenin (Ser45; 1:1,000; Cell Signalling Technology), anti-α-tubulin (1:1,000; Cell Signalling Technology), anti-α-tubulin (1:1,000; Cell Signalling Technology), anti-Cyclin D2 (1:1,000; Cell Signalling Technology), anti-Cyclin A (1:1,000; Abcam), anti-Cyclin B2 (1:1,000; Santa Cruz Biotechnology), anti-Mcm2 (1:1,000; Cell Signalling Technology), anti-Pbk (1:1,000; Cell Signalling Technology), anti-C-myc (1:1,000; Cell Signalling Technology), anti-GAPDH (1:10,000; KANGCHEN) and anti-lamin B (1:1,000; Santa Cruz Biotechnology). Images have been cropped for presentation. Full size images are presented in Supplementary Figs 9–11.

Anti-preS1 (Aprogen, Daejeon, Korea), anti-cyclin A, anti-cyclin D1, anti-cdk2, anti-cdk4, anti-PCNA (Abcam, Cambridge, UK), anti-p53 and anti-β-actin (Santa Cruz Biotechnology Santa Cruz, CA, USA) antibodies were used for immunoblotting.

Hepatocytes were isolated by two‐step collagenase digestion and gradient centrifugation. Total protein of hepatocytes was extracted using RIPA lysis buffer (Beyotime, People's Republic of China). A total of 15 μg of protein/well were electrophoresed by 10% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS‐PAGE). After the protein transfer was completed, the PDVF membranes were blocked with 5% non‐fat powdered milk at room temperature for 1 hours. Next, the PDVF membranes were incubated with anti‐Brg1 (1:10 000; Abcam), anti‐CDK1 (1:10 000; Abcam), anti‐CDK4 (1:2000; Abcam), anti‐cyclin A, (1:2000; Abcam), anti‐cyclin D1, (1:10 000; Abcam), anti‐cyclin E1, (1:1000; Abcam), anti‐LATS1, (1:5000; Abcam), anti‐p‐YAP, (1:5000; Abcam), anti‐YAP, (1:5000; Abcam) and anti‐GAPDH antibody (1:1000, Abcam) at 4°C overnight. GAPDH was used as an internal reference. Finally, enhanced chemiluminescence (ECL) (Thermo Fisher) was used to detect the expression of the target proteins.