Anti mouse polyclonal igg secondary antibodies

Manufactured by Santa Cruz Biotechnology

Anti-mouse polyclonal IgG secondary antibodies are used to detect and bind to primary antibodies raised against mouse antigens. They are designed to amplify the signal from the target primary antibody, enabling more sensitive detection and visualization of the target protein or analyte.

Automatically generated - may contain errors

Lab products found in correlation

Chemidoc imaging system, by Bio-Rad (2 mentions) Amersham ecl select, by GE Healthcare (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) Amersham ecl, by Cytiva (2 mentions) P akt ser473, by Cell Signaling Technology (1 mentions) P akt thr450, by Cell Signaling Technology (1 mentions) Gsk3β, by Cell Signaling Technology (1 mentions) Maplc3, by Cell Signaling Technology (1 mentions) P70s6k thr389, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Polyclonal antibodies (15 citations) Anti-mouse secondary antibodies (12 citations) Anti-mouse antibodies (7 citations) Mouse secondary antibodies (6 citations) IgG mouse (6 citations) Mouse polyclonal antibodies (3 citations) Anti-IgG antibodies (3 citations) Mouse IgGs (3 citations) Mouse IgG antibodies (2 citations) Mouse anti-IgG (2 citations)

3 protocols using anti mouse polyclonal igg secondary antibodies

Phosphorylation of AKT and mTOR Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols

Phosphorylation of AKT and downstream targets of AKT and mTOR pathway with or without SC-66 (6–10 µg/ml) and MK-2206 (0–2.5 µM) were determined by western blotting with primary antibodies against phosphorylated and total forms of mTOR, p70s6k, 4E-BP1, S6, GSK3-β, FOXO pAKT^Thr308, pAKT^Thr450 and pAKT^Ser473 (1∶1000; Cell Signaling Technology, MA), total forms of AKT, mTOR and 4-EBP1 (1∶1000, Cell Signaling Technology, MA), total forms of p70s6k and β-Actin HRP from Santa Cruz Biotechnology, CA and total forms of PRAS40 and FOXO from millipore (1∶5000, Santa Cruz Biotechnology,CA). β-Actin was used as the internal control. Blots were probed with HRP-conjugated anti-rabbit (Cell Signaling Technology, Beverly, MA) or anti-mouse polyclonal IgG secondary antibodies (Santa Cruz Biotechnology, CA) for 1 h at RT. For detection Pierce West Dura substrate (Pierce Biotechnology) was used according to manufacturer's protocol and exposed on X-ray film.

Rashmi R., DeSelm C., Helms C., Bowcock A., Rogers B.E., Rader J., Grigsby P.W, & Schwarz J.K. (2014). AKT Inhibitors Promote Cell Death in Cervical Cancer through Disruption of mTOR Signaling and Glucose Uptake. PLoS ONE, 9(4), e92948.

+ Open protocol

+ Expand

AMPK Pathway Activation and Autophagy

Check if the same lab product or an alternative is used in the 5 most similar protocols

Phosphorylation of AMPK pathway and activation of autophagy with or without 2-DG+BSO+AUR were determined by western blotting with primary antibodies against phosphorylated and total forms of ^Ser15p53, p53, ^{Thr269/Ser272}p62, p62, ^Thr389p70, p70, ^Ser317Ulk, Ulk, ^Ser62Myc, Myc, cleaved caspase 3 and PARP, ^Thr286CamKII, CamKII, ^Thr172AMPK, AMPK, ^Ser428LKB1, LKB1, ^{Thr183/Tyr185} JNK, JNK (1:1000; Cell Signaling Technology, MA), for total forms of thioredoxin reductase1 and 2 (TR1 and TR2), GAPDH, MAPLC3, and Bim (1:1000, Cell Signaling Technology, MA), and for Actin from (1:1000,Santa Cruz Biotechnology, CA) and tubulin (1:10,000, Sigma, Saint Louis, MO). Blots were probed with HRP-conjugated anti-rabbit (Cell Signaling Technology, Beverly, MA) or anti-mouse polyclonal IgG secondary antibodies (Santa Cruz Biotechnology, CA) for 1h at room temperature. For detection, Amersham ECL select (GE healthcare, PA) was used according to manufacturer’s protocol. Images were acquired using Chemidoc Imaging systems (BioRad).

Rashmi R., Huang X., Floberg J.M., Elhammali A.E., McCormick M.L., Patti G.J., Spitz D.R, & Schwarz J.K. (2018). Radio-resistant cervical cancers are sensitive to inhibition of glycolysis and redox metabolism. Cancer research, 78(6), 1392-1403.

+ Open protocol

+ Expand

Western Blotting Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blotting was performed with primary antibodies against phosphorylated and total forms of p70s6k ^Thr389 and Akt^Ser473 (1:1000; Cell Signaling Technology, MA), for total forms of PTEN (1:1000; Cell Signaling Technology, MA), GAPDH, Akt (1:1000, Cell Signaling Technology, MA), and for total forms of p70s6k and Actin (1:1000, Santa Cruz Biotechnology, CA) and PC (1:500, Thermo Fisher, MA). Blots were probed with HRP-conjugated anti-rabbit (Cell Signaling Technology, Beverly, MA) or anti-mouse polyclonal IgG secondary antibodies (Santa Cruz Biotechnology, CA) for 1 h at room temperature. For detection, Amersham ECL select (GE-healthcare, PA) was used according to manufacturer’s protocol. Images were acquired using Chemidoc Imaging systems (BioRad).

Rashmi R., Jayachandran K., Zhang J., Menon V., Muhammad N., Zahner M., Ruiz F., Zhang S., Cho K., Wang Y., Huang X., Huang Y., McCormick M.L., Rogers B.E., Spitz D.R., Patti G.J, & Schwarz J.K. (2020). Glutaminase inhibitors induce thiol-mediated oxidative stress and radio-sensitization in treatment resistant cervical cancers. Molecular cancer therapeutics, 19(12), 2465-2475.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!