Naoh beads

Manufactured by Merck Group

Sourced in United States

NaOH beads are a laboratory product manufactured by Merck Group. They are a form of sodium hydroxide, a common inorganic chemical compound. NaOH beads are used in various laboratory applications that require a source of hydroxide ions.

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Lab products found in correlation

Formic acid, by Thermo Fisher Scientific (3 mentions) Chloroform, by Merck Group (2 mentions) Iodomethane, by Merck Group (2 mentions) Hplc grade methanol, by Thermo Fisher Scientific (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Ammonium borane complex, by Merck Group (2 mentions) Absolute ethanol, by Merck Group (2 mentions) Cell counting kit 8 cck 8, by Beyotime (2 mentions) Acetone, by Merck Group (2 mentions) Cacl2 solution, by Merck Group (2 mentions) Live dead viability cytotoxicity kit, by Thermo Fisher Scientific (2 mentions) Acetonitrile, by Merck Group (1 mentions) Peptide n glycosidase f, by Merck Group (1 mentions) Formic acid, by Mallinckrodt (1 mentions) C18 micro spin columns, by Harvard Apparatus (1 mentions) Empty micro spin columns, by Harvard Apparatus (1 mentions) Gemini c18 column, by Phenomenex (1 mentions) Sep pak c18 cartridge, by Waters Corporation (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions) Iodoacetamide, by Merck Group (1 mentions)

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Sodium hydroxide (NaOH) beads (4 citations)

6 protocols using naoh beads

Glycoproteomics Analysis of Ribonuclease B

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Ribonuclease B from bovine pancreas, peptide:N-glycosidase F (PNGase F), chloroform, NaOH beads (97% purity), iodomethane, 2-mercaptoethonal, acetonitrile (ACN), and all metal acetates were purchased from Sigma-Aldrich (St. Louis, MO, USA). C18 micro spin columns and empty micro spin columns were from Harvard Apparatus (Holliston, MA, USA). Water (HPLC grade) was obtained from EMD Chemicals (Darmstadt, Germany). Dimethylformamide (DMF), trifluoroacetic acid (TFA) and formic acid were obtained from Mallinckrodt Baker (Phillipsburg, NJ, USA).

Zhu F., Glover M.S., Shi H., Trinidad J.C, & Clemmer D.E. (2014). Populations of Metal-Glycan Structures Influence MS Fragmentation Patterns. Journal of the American Society for Mass Spectrometry, 26(1), 25-35.

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Glycoprotein Purification and Analysis

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Toyopearl AF-Formyl-650M resin was from Tosoh Bioscience (King of Prussia, PA, USA). Wheat germ agglutinin (WGA) was purchased from Vector Laboratories (Burlingame, CA, USA). Stainless steel columns (2×250 mm) and the Gemini C18 column were obtained from Phenomenex (Torrance, CA, USA). Ovomucoid (type III-O, free of ovoinhibitor), trypsin (TPCK treated), PNGase F, chloroform, NaOH beads (97% purity), iodomethane, 2-mercaptoethanol, dithiothreitol, iodoacetamide, trizma base, and sodium cyanoborohydride (NaCNBH₃) were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Water and acetonitrile (ACN) were obtained from EMD Chemicals (Darmstadt, Germany). Formic acid was from Alfa Aesar (Ward Mill, MA, USA). Trifluoroacetic acid (TFA), dimethylformamide (DMF), urea, ammonium bicarbonate, sodium chloride, magnesium chloride, and calcium chloride were all from Mallinckrodt Pharmaceuticals (St. Louis, MO, USA). C18 Zip-tips were from Agilent Technologies (Santa Clara, CA, USA). C18 Sep-Pak cartridges were from Waters (Milford, MA, USA). Empty spin columns were purchased from Harvard Apparatus (Holliston, MA, USA).

Zhu F., Trinidad J.C, & Clemmer D.E. (2015). Glycopeptide Site Heterogeneity and Structural Diversity Determined by Combined Lectin Affinity Chromatography/IMS/CID/MS Techniques. Journal of the American Society for Mass Spectrometry, 26(7), 1092-1102.

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Glycoprotein N-Deglycosylation Protocol

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PNGase F, G7 buffer (50mM sodium phosphate buffer, pH 7.5) were from New England Biolabs (Ipswich, MA, USA). Ammonium-borane complex, CH₃I iodomethane, Dimethyl sulfoxide (DMSO), NaOH beads were purchased from Sigma-Aldrich (St. Louis, MO, USA), ethanol was from PHARMCO-AAPER, HPLC grade methanol, Acetonitrile (ACN), Formic Acid, HPLC water were obtained from Fisher Scientific (Fair Lawn, New Jersey, USA).

Dong X., Mondello S., Kobeissy F., Talih F., Ferri R, & Mechref Y. (2018). LC-MS/MS Glycomics of Idiopathic Rapid Eye Movement Sleep Behavior Disorder. Electrophoresis, 39(24), 3096-3103.

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Cell Viability and ROS Assay Protocol

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Dulbecco’s phosphate-buffered saline (DPBS, without calcium and magnesium) was purchased from Gibco (Paisley, PA4, UK). CaCl₂ solution, NaOH beads, acetone and absolute ethanol were obtained from Sigma-Aldrich (St. Louis, MO, USA). VAP and VAP-FITC were synthesized by Mingze Biotechnology (Xian, China). Micro BCA protein assay kit (mBCA), rhodamine-conjugated phalloidin and live/dead viability/cytotoxicity kit were obtained from Thermo Scientific (Rockford, IL, USA). RSC96 cells were obtained from Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Phosphate-Buffered Saline (PBS), Penicillin–Streptomycin (Pen-Strep), Fetal Bovine Serum (FBS) and Dulbecco’s Modified Eagle’s medium (DMEM) were purchased from Hyclone (Logan, UT, USA). 0.25% trypsin–EDTA, 4% paraformaldehyde solution, Triton X-100, nuclei visualization solution DAPI (6-diamidino-2-phenylindole), Cell Counting Kit-8 (CCK-8) and reactive oxygen species assay kit were obtained from Beyotime Biotechnology (Shanghai, China). All cell culture dishes and multi-well plates were purchased from Corning (NY, USA).

Mao R., Du D., Zhu X, & Li W. (2022). Velvet antler polypeptide combined with calcium phosphate coating to protect peripheral nerve cells from oxidative stress. Journal of Molecular Histology, 53(6), 915-923.

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Optimizing Neural Stem Cell Culture

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Dulbecco’s phosphate-buffered saline (DPBS, without calcium and magnesium), phosphate-buffered saline (PBS), accutase cell dissociation reagent, micro BCA protein assay kit (mBCA), serum albumin and live/dead viability/cytotoxicity kit were purchased from Thermo Fisher Scientific (Waltham, MA, USA). LN from murine, CaCl₂ solution, NaOH beads, acetone, absolute ethanol, and glutaraldehyde were obtained from Sigma-Aldrich (St Louis, MO, USA). Fluorescein isothiocyanate-conjugated LN (FITC-LN) was synthesized by Solarbio (Beijing, People’s Republic of China). NSC derived from brain tissues of Sprague Dawley (SD) rat embryos and NSC growth medium were obtained from Cyagen Biosciences (Soochow, People’s Republic of China). Mouse monoclonal anti-nestin was purchased from Abcam (Cambridge, UK). Secondary antibodies for immunofluorescence staining, Triton X-100, nuclei visualization solution DAPI, and Cell Counting Kit-8 (CCK8) were obtained from Beyotime Biotechnology (Shanghai, People’s Republic of China). All cell culture flask and multi-well plates were purchased from Corning Incorporated (Corning, NY, USA).

Luo D., Ruan S., Liu A., Kong X., Lee I.S, & Chen C. (2018). Laminin functionalized biomimetic apatite to regulate the adhesion and proliferation behaviors of neural stem cells. International Journal of Nanomedicine, 13, 6223-6233.

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Methylation Analysis of Glycoproteins

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Ribonuclease B (RNase B) and Fetuin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Intact mAb was acquired from Waters Corporation. PNGase F and G7 buffer (50mM sodium phosphate buffer, pH 7.5) were from New England Biolabs (Ipswich, MA, USA). Ammonium-borane complex, CH₃I, CH₂DI, CHD₂I, CD₃I, ¹³CH₃I, ¹³CH₂DI, ¹³CHD₂I, ¹³CD₃I iodomethane reagents, Dimethyl sulfoxide (DMSO), and NaOH beads were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ethanol was obtained from PHARMCO-AAPER. HPLC-grade methanol, Acetonitrile (ACN), Formic Acid, and HPLC water were obtained from Fisher Scientific (Fair Lawn, New Jersey, USA).

Dong X., Peng W., Yu C.Y., Zhou S., Donohoo K.B., Tang H, & Mechref Y. (2019). 8-plex LC-MS/MS Analysis of Permethylated N-glycans Achieved by Using Stable Isotopic Iodomethane. Analytical chemistry, 91(18), 11794-11802.

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