Hoechst33342

Skipjack tuna roes were provided by Ningbo Today Food Co., Ltd. (Zhejiang, China). HUVECs were purchased from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). ROS (Product no. E004-1-1), Hoechst33342 (Product no. G023-1-1), CAT (Product no. A007-1-1), SOD (Product no. A001-3-2), GSH-PX (Product no. A005-1-2), MDA (Product no. A003-1-2), ET-1 (Product no. H093-1-2), and NO (Product no. A013-2-1) kits were purchased from Nanjing Jiancheng Bioengineering Institute (Jiangsu, China). Glutathione (GSH), trypsin, pepsin, papain, ACE and FAPGG were purchased from Sigma-Aldrich (Shanghai) Trading Co., Ltd. (China). Alcalase, MTT, DMEM, DMSO, fetal bovine serum (FBS), norepinephrine (NE), trifluoro acetic acid (TFA), neutrase and Cap were purchased from Beijing Solarbio Science & Technology Co., Ltd. (China). ACEi peptides of WGESF (TRP3), IKSW (TRP6), YSHM (TRP9), and WSPGF (TRP12) with purity higher than 95% were synthesized in Shanghai Apeptide Co. Ltd. (China).

Primary chicken liver cells were cultured on a glass slide in 24-well plates. The cells were washed with PBS and then stained with Hoechst 33342 (1 mg/mL, Nanjing Jiancheng, China) for 10 min. After two washes with PBS, the cells were examined using fluorescence microscopy.