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2 protocols using boric acid

1

Synthesis of Homemade TiO2 Photocatalysts

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Titanium-isopropoxide (≥97.0%, Sigma-Aldrich Inc., St. Louis, MO, USA) was used for the synthesis of homemade TiO2. Aerolyst® TiO2 (P25, Evonik, Essen, Germany) was also used as semiconductor. The Pt(NH3)4(NO3)2 platinum precursor was supplied by Sigma-Aldrich Inc. (St. Louis, MO, USA). Glycerol (99%), 2-propanol and HNO3 (65%) were products of Molar Chemicals Ltd. (Budapest, Hungary. Hydrofluoric acid (38%) and boric acid were products of Reanal (Budapest, Hungary). Absolute ethanol was purchased from VWR International (Fontenay-sous-Bois, France). Double distilled water (18 MΩ) was used for the synthesis of photocatalysts and for preparation of Glycerol solution. The gases (H2, N2, Ar) used in this work were products of Linde Gáz Magyarország Zrt. (Budapest, Hungary) with 5.0 purity. Special mixture of 5% H2 in N2 for calibration of GC was bought from Messer Hungarogáz Ltd. (Budapest, Hungary).
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2

Quantification of Glucosinolates and Isothiocyanates

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For all applications, bidistilled water was used. The used chemicals listed below were all of at least analytical grade. Boric acid, monosodium phosphate monohydrate, ascorbic acid, sodium hydroxide and hydrochloric acid were obtained from Reanal (Budapest, Hungary). Acetic acid, Ncyclohexyl-ethanesulphonic acid (CHES), acetonitrile, mercaptoAcetic acid (thioglycolic acid) were bought from VWR Hungary (Budapest, Hungary). Sodium deoxycholate was from Carl-Roth (Karlsruhe, Germany). Sinigrin and gluconasturtiin were from Phytoplan (Heidelberg, Germany). Allyl isothiocyanate, phenethyl isothiocyanate, bovine serum albumine and Bradford reagent were from Sigma-Aldrich (St.Louis, MO, USA).
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