Cytokine measurements in the various figures were performed at different time intervals, which are indicated in the legends for the respective figures.
Concentrations of mouse TNF and IL-6 produced by bone marrow-derived cultured CD49b+ cells were measured using the ProcartaPlex Multiplex Immunoassay (Essential Th1/Th2, 6plex, catalog number EPX060–20831-901, Fisher Scientific) with detection limits of 0.39 pg/ml and 0.21 pg/ml, respectively. Concentrations of TNF produced by basophils isolated from the bone marrow were measured with a high sensitivity ELISA kit from eBioscience (catalog number BMS607HS) with a detection limit of 0.75 pg/ml.
Concentrations of TNF, IL-6, KC, and MIP-2 (for serum and intraperitoneal lavage fluids) were measured with ELISAs. The TNF and IL-6 ELISAs were from BioLegend (catalog numbers 430904 and 431304, respectively); and the KC and MIP-2 ELISAs were from Peprotech (catalog numbers 900-K127 and 900-K152, respectively). The detection limits for these assays were 4 pg/ml for TNF, 2 pg/ml for IL-6, 4 pg/ml for KC and 16 pg/ml for MIP-2.
Concentrations of mouse TNF and IL-6 produced by bone marrow-derived cultured CD49b+ cells were measured using the ProcartaPlex Multiplex Immunoassay (Essential Th1/Th2, 6plex, catalog number EPX060–20831-901, Fisher Scientific) with detection limits of 0.39 pg/ml and 0.21 pg/ml, respectively. Concentrations of TNF produced by basophils isolated from the bone marrow were measured with a high sensitivity ELISA kit from eBioscience (catalog number BMS607HS) with a detection limit of 0.75 pg/ml.
Concentrations of TNF, IL-6, KC, and MIP-2 (for serum and intraperitoneal lavage fluids) were measured with ELISAs. The TNF and IL-6 ELISAs were from BioLegend (catalog numbers 430904 and 431304, respectively); and the KC and MIP-2 ELISAs were from Peprotech (catalog numbers 900-K127 and 900-K152, respectively). The detection limits for these assays were 4 pg/ml for TNF, 2 pg/ml for IL-6, 4 pg/ml for KC and 16 pg/ml for MIP-2.