Log In Sign up
The largest database of trusted experimental protocols

Mouse monoclonal testosterone antibody clone 4e1g2

Manufactured by Bio-Rad
Visit Supplier
Sourced in United States

The Mouse monoclonal testosterone antibody (clone 4E1G2) is a laboratory reagent designed for immunoassay applications. It is a mouse-derived monoclonal antibody specifically targeted against the testosterone molecule.

Automatically generated - may contain errors

Lab products found in correlation

Rabbit polyclonal progesterone antibody, by Bio-Rad (3 mentions) Tritium labelled radioactive progesterone, by PerkinElmer (2 mentions) Testosterone, by PerkinElmer (1 mentions) Luteinizing hormone lh, by Merck Group (1 mentions) Follicle stimulating hormone fsh, by Merck Group (1 mentions) Adrenocorticotropic hormone acth, by Merck Group (1 mentions)

3 protocols using mouse monoclonal testosterone antibody clone 4e1g2

1

Steroid Hormones Quantification by RIA

Check if the same lab product or an alternative is used in the 5 most similar protocols
Progesterone and testosterone secretion was measured in cell supernatant by radioimmunoassay (RIA). Tritium-labeled radioactive progesterone and testosterone were obtained from Perkin Elmer (Waltham, MA, USA). Mouse monoclonal testosterone antibody (clone 4E1G2) and rabbit polyclonal progesterone antibody were procured from Bio-Rad (Hercules, CA, USA) and used at a dilution of 1:500.
Goyal A.K, & Saini J. (2018). Steroidogenesis and VEGF Production Doesn't Alter in Leydig Cells within the Homeostatic Range of Testicular Temperature. Journal of Human Reproductive Sciences, 11(3), 291-296.
+ Open protocol
+ Expand
2

Steroidogenic Pathway Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pituitary-derived TSH, Luteinizing hormone (LH), Follicle stimulating hormone (FSH) and Adrenocorticotropic hormone (ACTH) were purchased from Sigma–Aldrich (St. Louis, USA). Recombinant TSH was purchased from R&D systems (Minneapolis, USA). Tritium labelled radioactive progesterone and testosterone were purchased from Perkin Elmer (Waltham, USA). Both the mouse monoclonal testosterone antibody (clone 4E1G2) and rabbit polyclonal progesterone antibody were purchased from Bio-Rad (Hercules, USA). Primers were purchased from Sigma–Aldrich (St. Louis, USA). Rabbit anti-StAR antibody and rabbit anti-CYP11A1 antibody were purchased from Cell Signalling Technologies (Boston, USA). Rabbit anti-3β-hydroxy steroid dehydrogenase-1 (3BHSD-1) antibody and rabbit anti-TSH receptor antibody were purchased from Abcam (Cambridge, UK). Anti-rabbit HRP labelled secondary antibody was purchased from Cell Signalling Technologies (Boston, USA).
Dhole B., Gupta S., Shekhar S, & Kumar A. (2020). A Novel Antigonadotropic Role of Thyroid Stimulating Hormone on Leydig Cell-Derived Mouse Leydig Tumor Cells-1 Line. Annals of the National Academy of Medical Sciences (India), 56(1), 30-37.
+ Open protocol
+ Expand
3

Steroid Hormone Quantification by RIA

Check if the same lab product or an alternative is used in the 5 most similar protocols
Progesterone and testosterone secretion was measured in cell supernatant by radioimmunoassay (RIA) according to the WHO guidelines (Sufi et al. 1993) . Tritium labelled radioactive progesterone and testosterone were obtained from Perkin Elmer (Waltham, MA, USA). Mouse monoclonal testosterone antibody (clone 4E1G2) and rabbit polyclonal progesterone antibody were procured from Bio-Rad (Hercules, CA, USA) and used at a dilution of 1:500. The progesterone antibody used for RIA was highly specific and showed no cross-reactivity for pregnenolone or for testosterone. However, the testosterone antibody also recognizes 5αdihydrotestosterone (DHT, 1%) and androstenedione (1%). The intra-assay variation was less than 5% and the inter-assay variation was less than 10%.
Dhole B., Gupta S., Venugopal S.K, & Kumar A. (2018). Triiodothyronine stimulates VEGF expression and secretion via steroids and HIF-1α in murine Leydig cells. Systems biology in reproductive medicine, 64(3).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!