Goat anti m c3

Manufactured by MP Biomedicals

Goat anti-M C3 is a laboratory reagent used for the detection and quantification of the C3 complement protein in various biological samples. It is produced by immunizing goats with the C3 antigen. The product can be used in immunoassays, such as ELISA, to measure C3 levels in research or clinical settings.

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Lab products found in correlation

Goat anti rabbit igg, by GE Healthcare (1 mentions) Rabbit anti human igg, by Jackson ImmunoResearch (1 mentions) Donkey anti sheep igg, by R&D Systems (1 mentions) Hrp conjugated goat anti rabbit igg, by Merck Group (1 mentions) Rabbit anti mouse igg hrp, by Agilent Technologies (1 mentions) Goat anti rat hrp, by BioLegend (1 mentions)

Spelling variants of this product

Anti-C3 (3 citations)

2 protocols using goat anti m c3

Quantitative Western Blotting of Mouse Complement Proteins

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Fresh serum from mice was employed to measure C3, FB, P or FD by Western
blotting. Goat anti-M C3 (MP Biomedicals), goat anti-Hu FB (CompTech), rabbit
anti-M P (37 (link)), sheep anti-M FD
(R&D) and rabbit anti-Hu FD (Abcam, Cambridge, MA) were incubated with
the membranes for 2 h or overnight at 4° at RT. Secondary HRP-conjugated
rabbit anti-goat IgG (Sigma-Aldrich), goat anti-rabbit IgG (GE Healthcare UK
Limited), donkey anti-sheep IgG (R&D) or rabbit anti-human IgG (Jackson
Immuno Research) was added for 1 h at 37°C. Membranes were developed
with a SuperSignal West Kit (Pierce).

Wu X., Hutson I., Akk A.M., Mascharak S., Pham C.T., Hourcade D.E., Brown R., Atkinson J.P, & Harris C.A. (2018). Contribution of Adipose-derived Factor D/Adipsin to Alternative Pathway Complement Activation: Lessons from Lipodystrophy. Journal of immunology (Baltimore, Md. : 1950), 200(8), 2786-2797.

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Western Blotting of Complement Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples from individual mice were separated using SDS-PAGE under reducing conditions for mC3 and non-reducing conditions for mC5, hFH and mFH. Goat anti-mC3 (MP Biomedicals), goat anti-hC5 (Quidel), rat anti-mFH (R&D Systems) or goat anti-hFH (Quidel) were used as detection antibodies followed by goat anti-rat-HRP (Biolegend), mouse anti-goat/sheep IgG-HRP (Sigma) and rabbit anti-mouse IgG-HRP (Dako), as required. Blots were visualized using ECL Western Blotting Substrate (Pierce, Thermo).

Kamala O., Malik T.H., Hallam T.M., Cox T.E., Yang Y., Vyas F., Luli S., Connelly C., Gibson B., Smith-Jackson K., Denton H., Pappworth I.Y., Huang L., Kavanagh D., Pickering M.C, & Marchbank K.J. (2021). Homodimeric Minimal Factor H: In Vivo Tracking and Extended Dosing Studies in Factor H Deficient Mice. Frontiers in Immunology, 12, 752916.

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