Cytokine chemokine 9 plex porcine procartaplex panel 1

Plasma samples were taken at baseline, after 30 min and every 60 min after LPS installation. BALF samples were obtained at baseline and the end with an Ambu® aScope^TM (Ambu). Plasma and BALF were analyzed with the cytokine multiplex kit Cytokine & Chemokine 9‐Plex Porcine ProcartaPlex™ Panel 1 (Thermo Fisher Scientific Cat. No. EPX090‐60829–901) according to the manufacturer's instructions. The kit was analyzed using a Bioplex‐200 system (BioRad). The six cytokines in the kit included: IL‐1β, IL‐6, IL‐8, IL‐10, IL‐12, and TNF‐α.

Measurements of cytokine levels in the plasma were taken at baseline, and every 60 min. Bronchoalveolar lavage fluid (BALF) was taken at baseline and at the end of the experiment. IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p40, IFN-γ and TNF-α levels were evaluated using xMAP technology with a Luminex 200 system according to manufacturer’s instructions (n = 12). IFN-α levels (n = 9) were analysed with the multiplex kit Cytokine & Chemokine 9-Plex Porcine ProcartaPlex™ Panel 1 (Thermo Fisher Scientific Cat. No. EPX090-60829-901, Waltham, MA, USA) according to the manufacturer’s instructions, using a Bioplex-200 system (BioRad, Hercules, CA, USA).

Measurements of cytokine levels in the plasma were taken at baseline, every 60 min in the donor animals, every hour during EVLP, and every 4th hour in the recipient animal. These levels were analyzed with the multiplex kit Cytokine & Chemokine 9-Plex Porcine ProcartaPlex™ Panel 1 (Thermo Fisher Cat. No. EPX090-60829-901, Thermo Fisher Scientific, Waltham, Massachusetts, US) according to the manufacturer’s instructions. The kit was run using a Bioplex-200 system (BioRad, Hercules, CA, USA). Nine cytokines were evaluated: IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p40, IFN-α, IFN-γ and TNF-α. Bronchoalveolar lavage fluid (BALF) was taken at baseline in donor animals, before lung harvest in donor animals, at the end of the EVLP, and at the end of the experiment.