A2780 cell line was purchased from Sigma-Aldrich. 2008 cell line was kindly provided by Dr. François X. Claret (University of Texas M. D. Anderson Cancer Center). RMG-1 and HCH-1 were a kind gift of Dr. Hiroaki Itamochi (Tottori University, Japan). All other cell lines were purchased from the American Type Culture Collection and were maintained in either DMEM (SKOV-3, OVCAR-3, OVCAR-8, CAOV-3 and IGROV-1), McCoy's 5a Medium Modified (HT-29 and ES-2) or RPMI-1640 (2008, RMG-1, ECC-1 and HCH-1), supplemented with 10% fetal calf serum (or 20% for OVCAR-3), penicillin (100 units/mL), and streptomycin (100 μg/mL) at 37°C with 5% CO2 in a humidified incubator. Reagents were obtained as follows: Trypsin (Sigma-Aldrich cat. #: T1426). HE4 (Fujirebio); SLIGKV-NH2 (Tocris cat. #: 3010); A2M (Prospec cat. #: PRO-551); TATI (R&D systems cat. #: 7496-PI); SLPI (R&D systems cat. #: 1274-PI); PAR-2 Antibody (Santa Cruz Biotechnology cat. #: sc-13504); ERK and phospho-ERK antibodies (Cell Signaling Technology cat. #: 9102 and 4370 respectively).
Erk and phospho erk antibodies
Manufactured by Cell Signaling Technology
Sourced in United States
ERK and phospho-ERK antibodies are lab equipment products manufactured by Cell Signaling Technology. These antibodies are used to detect and quantify the expression of ERK and its phosphorylated forms in biological samples. The core function of these antibodies is to provide researchers with tools to study the activation and regulation of the ERK signaling pathway, which is involved in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Skov3, by American Type Culture Collection (1 mentions)
Ovcar3, by American Type Culture Collection (1 mentions)
Trypsin, by Merck Group (1 mentions)
A2780 cell line, by Merck Group (1 mentions)
Rmg 1, by American Type Culture Collection (1 mentions)
Par 2 antibody, by Santa Cruz Biotechnology (1 mentions)
Sligkv nh2, by Bio-Techne (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Mk 801, by Merck Group (1 mentions)
Gapdh antibody, by Santa Cruz Biotechnology (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Poly l lysine (pll), by Merck Group (1 mentions)
Insulin, by Merck Group (1 mentions)
Nifedipine, by Merck Group (1 mentions)
Transferrin, by Merck Group (1 mentions)
Cytosine arabinoside, by Merck Group (1 mentions)
Imperatorin, by ChromaDex (1 mentions)
Diltiazem, by Merck Group (1 mentions)
γ aminobutyric acid, by Merck Group (1 mentions)
2 protocols using erk and phospho erk antibodies
1
Characterization of Ovarian Cancer Cell Lines
2
Imperatorin: Cellular Signaling Modulator
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Imperatorin was purchased from ChromaDex (Irvine, CA, USA). Dulbecco's modified eagle's medium (DMEM) and fetal bovine serum (FBS) were obtained from Invitrogen (Carlsbad, CA, USA). Insulin, transferrin, γ-amino butyric acid, poly-L-lysine, cytosine arabinoside, MK801, diltiazem, and nifedipine were from Sigma-Aldrich (St. Louis, MO, USA). The ERK and phospho-ERK antibodies were from Cell Signaling Technology (Beverly, MA) and GAPDH antibody was from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
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