Amersham films

Manufactured by GE Healthcare

Amersham films are a line of medical imaging films produced by GE Healthcare. These films are designed for use in various imaging modalities, including X-ray, mammography, and other medical imaging procedures. The core function of Amersham films is to capture and record medical images for diagnostic and treatment purposes.

Automatically generated - may contain errors

Lab products found in correlation

Anti ve cadherin antibody, by Cell Signaling Technology (1 mentions) A2547, by Merck Group (1 mentions) A5228, by Merck Group (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) Ab167453, by Abcam (1 mentions) Ab1218, by Abcam (1 mentions) Gfp trap kit, by Proteintech (1 mentions) Goat anti rabbit hrp, by Thermo Fisher Scientific (1 mentions) Lipofectamine, by Thermo Fisher Scientific (1 mentions) 4 to 20 gradient gel, by Bio-Rad (1 mentions) Jetprime reagent, by Polyplus Transfection (1 mentions)

2 protocols using amersham films

Protein Expression Analysis of Aortic Endothelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Aortic EC protein extracts were obtained from cell confluent monolayers that had been exposed or not to recombinant human TGFbeta (5ng/ml; R and D systems) using Laemmli buffer. Proteins were separated by SDS-PAGE and transferred after electrophoresis onto a PVDF membrane (Millipore). Samples were blocked in 5% (w/v) BSA in TBS/Tween 0.1% (w/v). The membrane was probed with either a polyclonal antibody against eNOS at 1/1000 (Cell Signaling Technology, #9572), a polyclonal anti-VE-cadherin antibody at 1/100 (Cell Signalling Technology, #2500), a polyclonal anti-CD31 antibody at 0.4 μg/ml (Santa Cruz, sc-1506), a monoclonal anti-smooth muscle myosin heavy chain (SM-MHC) antibody at 1μg/ml (Chemicon, MAB3570), a monoclonal anti-alpha smooth muscle actin (alphaSMA) antibody at 1/2000 or 2.5 μg/ml (Sigma, A5228 or A2547) or a monoclonal anti-tubulin antibody at 0.2 μg/ml (Sigma, T6074). In a second step, the species corresponding to horseradish peroxidase-conjugated secondary antibodies (from Jackson ImmunoResearch Laboratories Inc) were applied. The immunoreactions were developed using the Amersham ECL^TM (Enhanced Chemi-luminescence) Western blotting detection reagents (GE Healthcare) and the membranes treated with the detection reagent were exposed to Amersham films (GE Healthcare).

Leclercq A., Veillat V., Loriot S., Spuul P., Madonna F., Roques X, & Génot E. (2015). A Methodology for Concomitant Isolation of Intimal and Adventitial Endothelial Cells from the Human Thoracic Aorta. PLoS ONE, 10(11), e0143144.

+ Open protocol

+ Expand

Protein-Protein Interaction Assay in HEK293 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following constructs were cotransfected in a 10-cm dish containing 30 to 40% confluent HEK293 cells using either Lipofectamine (Life Technologies) or jetPRIME reagent (Polyplus-transfection) following the manufacturer’s instructions: GFP-FAM161A with mCherry-POC5, mCherry-POC5 alone, GFP–Centrin-2 with mCherry-FAM161A, and GFP-POC5 with mcherry-POC1B. After 24 hours of expression, cells were processed according to the GFP-Trap Kit (Chromotek) instructions using radioimmunoprecipitation assay (RIPA) buffer for the lysis and incubating the lysate with GFP-Trap magnetic beads for 2 hours at 4°C on a rotating wheel. Samples were run on a 4 to 20% gradient gel (Bio-Rad) before transfer on polyvinylidene difluoride (PVDF) membrane using the iBlot system (Invitrogen/Thermo Fisher Scientific). Membranes were either incubated with primary antibodies against mouse GFP (1:700; Abcam, ab1218) or rabbit mCherry (1:500; Abcam, ab167453), followed by goat anti-mouse horseradish peroxidase (HRP) (1:1000 from a 50% glycerol stock; Thermo Fisher Scientific, 31431) or goat anti-rabbit HRP (1:1000 from a 50% glycerol stock; Thermo Fisher Scientific, 31468) secondary antibodies. Blots were developed using Amersham films (GE Healthcare, 28906836).

Le Guennec M., Klena N., Gambarotto D., Laporte M.H., Tassin A.M., van den Hoek H., Erdmann P.S., Schaffer M., Kovacik L., Borgers S., Goldie K.N., Stahlberg H., Bornens M., Azimzadeh J., Engel B.D., Hamel V, & Guichard P. (2020). A helical inner scaffold provides a structural basis for centriole cohesion. Science Advances, 6(7), eaaz4137.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!