The lysate from Hep3B and Huh7 cells was prepared using RIPA lysis buffer (radio-immunoprecipitation assay buffer), and the protein concentration was measured with Pierce™ BCA Protein Assay Kit (23,225, ThermoFisher, American). The level of SOX2 was detected with the anti-SOX2 antibody (2748, Cell signaling, American). HIF1A antibody (AF1935, R&D Systems, America), cleaved caspase3 antibody (8202S, Cell Signaling Technology, America) and PCNA antibody (GTX100539, GeneTex, China) were used in this study.
Pcna antibody
Manufactured by GeneTex
The PCNA antibody is a laboratory reagent used in biological research. It specifically binds to the Proliferating Cell Nuclear Antigen (PCNA) protein, which is a key component involved in DNA replication and repair processes.
Automatically generated - may contain errors
3 protocols using pcna antibody
1
Protein Expression Analysis in Liver Cancer Cells
2
Protein Expression Analysis in Liver Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The lysate from Hep3B and Huh7 cells was prepared using RIPA lysis buffer (radio-immunoprecipitation assay buffer), and the protein concentration was measured with Pierce™ BCA Protein Assay Kit (23225, ThermoFisher, American). The level of SOX2 was detected with the anti-SOX2 antibody (2748, Cell signaling, American). HIF1A antibody (AF1935, R&D Systems, America), cleaved caspase3 antibody (8202S, Cell Signaling Technology, America) and PCNA antibody (GTX100539, GeneTex, China) were used in this study.
3
Protein Expression Analysis in Liver Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The lysate from Hep3B and Huh7 cells were prepared using RIPA lysis buffer (radio-immunoprecipitation assay buffer), and the protein concentration was measured with Pierce™ BCA Protein Assay Kit (23225, ThermoFisher, American). Finally, the level of SOX2 was detected with the anti-SOX2 antibody (2748, Cell signaling, American). HIF1A antibody (AF1935, R&D Systems, America), cleaved caspase3 antibody (8202S, Cell Signaling Technology, America) and PCNA antibody (GTX100539, GeneTex, China) were used in this study.
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