Ethylene dimethacrylate

All chemicals were of analytical reagent grade. Ethylenedimethacrylate (EDMA, 99%), acetonitrile (LC-MS grade), and 3-trimethoxysilylpropyl methacrylate (TMSPM, 98%) were purchased from Sigma-Aldrich. 2.2′-Azobisizobutyronitrile (AIBN) and methanol (LC-MS grade) were purchased from Merck A.G (Darmstadt, Germany). A reducing union (1/16′ to 360 μm) SS, for FS tubing (S/N: L-P112240) from VICI Valco was used for the capillary connection between the monolithic column and the flow cell of detector. A TSP-050375-fused silica capillary with a 50 μm i.d. and a 363 μm o.d. (Lot: BUHT02A) was obtained from BGB Analytik. An amount of 2- propanol was purchased from Carlo Erba (for HPLC, isocratic grade). Ethanol (LC grade) was purchased from Isolab. Lauryl methacrylate (LMA, Lot: MKCL9070) and poly (ethyleneglycol) (PEG, Lot: BCCD8009) were supplied from Sigma-Aldrich. Coenzyme Q10 (≥98, HPLC, Lot: SLCF9505) was purchased from Sigma-Aldrich, and sodium chloride (NaCI) was supplied from Merck.

2,2-Dimethoxy-2-phenylacetophenone (DMPA), all functional monomers (acrylamide, AM; acrylonitrile, AN; 4-acryloylmorpholine, AMO; allylamine, ALA; N,N-diethylaminoethylmethacrylate, DEAEM; N,N-dimethylacrylamide, DMAM; N,N-dimethylaminoethylmethacrylate, DMAEM; ethylenglycol methacrylate phosphate, EGMP; 2-hydroxyethylmethacrylate, HEMA; methacrylic acid, MAA; methylacrylate, MA; monomethoxypolyethylenglycol 400 methacrylate, PEGMA; styrene, STY; 1-vinylimidazole, VIM; 4-vinylpyridine, 4VP; N-vinylpyrrolidone, NVP), cross-linkers (ethylene dimethacrylate, EDMA; glycerol dimethacrylate, GDMA; pentaerithrytole tetraacrylate, PETRA; trimethylolpropane trimethacrylate, TRIM) were from Sigma (Milan, Italy). Polymerization inhibitors eventually present in monomer solutions were removed by clean-up on activated alumina columns. Acetic acid, all organic solvents and all other chemicals were from VWR International (Milano, Italy). All the solvents were of HPLC grade, whereas all chemicals were of analytical grade. Patulin was from Fermentek (Jerusalem, Israel).
Patulin stock solutions were prepared by dissolving 10.0 mg of solid mycotoxin in 10 mL of acetonitrile and stored in the dark at −20 °C until use. Apple juice certified as free from patulin was a gift from Generon s.r.l. (Modena, Italy).

2,2′-Azobis-(2-methylpropionitrile), diclofenac sodium (DIC), ethylene dimethacrylate, mefenamic acid (MEF), and 4-vinylpyridine were purchased from Sigma-Aldrich-Fluka (Milan, Italy). Polymerization inhibitors were removed from the monomer solutions by clean-up on activated alumina. Acetic acid, acetonitrile, and methanol were purchased from VWR International (Milan, Italy). All the reagents used were of analytical grade. Diclofenac was transformed in its free acid form as follows: 250 mg of sodium salt was dissolved in 40 mL of deionized water. Then, the pH was adjusted to 1 using aqueous hydrogen chloride 1 M, and the turbid suspension was extracted three times using 15 mL of chloroform. The organic fractions were dried over anhydrous sodium sulphate and evaporated to dryness under reduced pressure, yielding the free acid quantitatively. Diclofenac and mefenamic acid stock solutions were prepared by dissolving 25 mg of the substance in 25 mL of acetonitrile then stored in the dark at −20 °C.

Ammonium oxalate, ferrous chloride tetrahydrate (FeCl₂·4H₂O), ferric chloride hexa-hydrate (FeCl₃·6H₂O), 2-hydroxyethyl methacrylate (HEMA), ethylene dimethacrylate (EDMA), 2,2′-azobis(2-methylpropionitrile) (AIBN), 2-dimethylaminoethyl methacrylate (DMAEMA), McCoy’s 5A medium, 4′,6-diamidino-2-phenylindol (DAPI), and Texas Red were purchased from Sigma-Aldrich (St. Louis, MI, USA). 1,4-Dioxan_, NH₄OH, and NaCl were obtained from Lach-Ner (Neratovice, Czech Republic). Other reagents were supplied from Lachema (Brno, Czech Republic). Q-water ultrafiltered on a Milli-Q Gradient A10 system (Millipore, Molsheim, France) water was used in the experiments.

All chemicals used in this experiment were of analytical reagent grade or higher. βCN and βLG were purchased from Sigma-Aldrich (Steinheim, Germany). Deionized water was obtained from the Mili-Q ultrapure water producing system (Millipore, Bedford, MA, USA). Trypsin Gold, Mass Spectrometry Grade used for digestion in a solution were provided by Promega (Madison, Wisconsin, USA). Fused-silica capillaries (150 μm i.d. × 375 μm o.d.) were purchased from CM Scientific Ltd. (Dublin, Ireland); 3-(trimethoxysilyl)propyl methacrylate (γ-MAPS), glycidyl methacrylate (GMA), ethylene dimethacrylate (EDMA), azobisisobutyronitrile (AIBN), 1-dodecanol, cyclohexanol, sodium bicarbonate, benzamidine, sodium cyanoborohydride, the storage solution (containing sodium azide), N-α-benzoyl-l-arginine ethyl ester (BAEE), trypsin from bovine pancreas, trifluoroacetic acid (TFA), acetonitrile (HPLC ultra-gradient grade), ammonium bicarbonate, dichloromethane, methanol, sodium hydroxide were purchased from Sigma–Adrich (Steinheim, Germany); acetone and toluene were purchased from Chempur (Poland); 1,6-hexanediamine, glutaraldehyde, sodium phosphate monobasic dihydrate were purchased from Alchem (Poland). All the chemicals supplied for the MALDI-TOF MS analyses were at the highest commercially available purity by Fluka Feinchemikalien (Neu-Ulm, Germany; a subsidiary of Sigma–Aldrich).