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Human il 12 p70 duoset elisa kit

Manufactured by R&D Systems
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The Human IL-12 p70 DuoSet ELISA kit is a reagent-based enzyme-linked immunosorbent assay (ELISA) used for the quantitative measurement of human interleukin-12 p70 in cell culture supernatants, serum, and plasma samples.

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Lab products found in correlation

Human ifn γ duoset elisa kit, by R&D Systems (1 mentions) Elisa kits for il 6, by BioLegend (1 mentions)

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DuoSet ELISA kits (1131 citations) DuoSet ELISA (635 citations) IL-12 (213 citations) DuoSets (49 citations) IL-12 ELISA kit (6 citations) Human IL-12 ELISA kit (4 citations) Human IL-12 (3 citations) Human IL-12 p70 DuoSet (2 citations) Human ELISA DuoSet (2 citations) Human kits (2 citations)

2 protocols using human il 12 p70 duoset elisa kit

1

Cytokine Quantification by ELISA

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Protein levels of IL-6, IL-12 p70, or IFN-γ in supernatants were evaluated using ELISA kits for IL-6 (Biolegend), IL-12 (Human IL-12 p70 DuoSet ELISA kit, R&D Systems), and IFN-γ (Human IFN-γ DuoSet ELISA kit, R&D Systems), following the manufacturer's instructions.
Laustsen A., Bak R.O., Krapp C., Kjær L., Egedahl J.H., Petersen C.C., Pillai S., Tang H.Q., Uldbjerg N., Porteus M., Roan N.R., Nyegaard M., Denton P.W, & Jakobsen M.R. (2018). Interferon priming is essential for human CD34+ cell-derived plasmacytoid dendritic cell maturation and function. Nature Communications, 9, 3525.
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2

Measuring IL-12 p40 and p70 in moDC Supernatants

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The supernatants from infected and stimulated moDC were collected and stored at –70 °C to later determine IL-12 p40 concentration. P40, without interference by IL-12 p70 protein, was determined with the capture antibody C8.3 for human IL-12p40, paired with the biotinylated C8.6 antibody as the detecting antibody, with recombinant human IL-12 p40 monomer as the standard BD (Franklin Lakes, New Jersey, USA). P70 was determined using a commercial kit (Human IL-12 p70 DuoSet® ELISA Kit (R&D Systems, Inc., Minneapolis, MN, USA) and following the manufacturer’s instructions.
Rodríguez-González J., Wilkins-Rodríguez A.A, & Gutiérrez-Kobeh L. (2024). Human Dendritic Cell Maturation Is Modulated by Leishmania mexicana through Akt Signaling Pathway. Tropical Medicine and Infectious Disease, 9(5), 118.
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