On the basis of morphological experiments, thrombi in the IVC of mice and the vessels themselves were collected for examination. According to the manufacturer's protocol, RNA was extracted with TRIzol Reagent at 4°C. RNA purity was determined using a NanoPhotometer spectrophotometer (IMPLEN) and the concentration was measured using a Qubit RNA Assay kit in a Qubit® 2.0 Fluorometer (Thermo Fisher Scientific, Inc.). RNA integrity was assessed using the RNA Nano 6000 Assay kit of the Bioanalyzer 2100 system (Agilent Technologies, Inc.). Then, RNA degradation and contamination were monitored on 1% agarose gels. Furthermore, RNA purity was assessed using the RNA Nano 6000 Assay kit of the Bioanalyzer 2100 system (Agilent Technologies, Inc.). Thus, qualified RNA was used as a material for later analyses and provided samples for RNA-seq.
Rna nano 6000 assay kit
Manufactured by Agilent Technologies
Sourced in United States, China, Germany, Canada
The RNA Nano 6000 Assay Kit is a laboratory equipment product designed for the analysis and quantification of RNA samples. It provides a standardized method for the assessment of RNA integrity and concentration.
Automatically generated - may contain errors
Lab products found in correlation
Bioanalyzer 2100 system, by Agilent Technologies (604 mentions)
Agilent bioanalyzer 2100 system, by Agilent Technologies (540 mentions)
Trizol reagent, by Thermo Fisher Scientific (416 mentions)
Nanophotometer spectrophotometer, by Implen (368 mentions)
Qubit rna assay kit, by Thermo Fisher Scientific (344 mentions)
Qubit 2.0 fluorometer, by Thermo Fisher Scientific (254 mentions)
Nanodrop 2000, by Thermo Fisher Scientific (199 mentions)
Nanophotometer, by Implen (161 mentions)
2100 bioanalyzer, by Agilent Technologies (124 mentions)
Agilent 2100 bioanalyzer, by Agilent Technologies (114 mentions)
Qubit 2.0 flurometer, by Thermo Fisher Scientific (105 mentions)
Nanodrop 2000 spectrophotometer, by Thermo Fisher Scientific (102 mentions)
Trizol, by Thermo Fisher Scientific (96 mentions)
Truseq pe cluster kit v3 cbot hs, by Illumina (94 mentions)
Novaseq platform, by Illumina (93 mentions)
Qubit rna assay kit in qubit 2.0 flurometer, by Thermo Fisher Scientific (93 mentions)
Ampure xp system, by Beckman Coulter (76 mentions)
Hiseq 2500 platform, by Illumina (65 mentions)
Hiseq platform, by Illumina (64 mentions)
Novaseq 6000, by Illumina (54 mentions)
1 496 protocols using rna nano 6000 assay kit
1
RNA Extraction from Murine Thrombosis
2
Transcriptome Sequencing of Plant Leaves
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In addition, transcriptome sequencing was performed on the above sample. Total RNA was extracted from the leaves using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instruction. RNA degradation and contamination were monitored on 1% agarose gels. RNA purity was checked using the Nanophotometer® spectrophotometer (IMPLEN, CA, USA). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). The RNA integrity was assessed using the RNA Nano 6000 Assay Kit (Agilent Technologies, Santa Clara, CA, USA). A total amount of 1 μg RNA per sample was used as input material for additional research [31 (link)].
3
RNA-seq Library Preparation and Sequencing
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Total RNA was isolated from the four sorted populations using RNAiso Plus (Takara Bio). RNA-seq was performed at the Beijing Novogene Bioinformatics Technology Co, using the Illumina NovaSeq platform. RNA Nano 6000 Assay Kits and the Bioanalyzer 2100 system (Agilent Technologies, CA, USA) were used to evaluate RNA integrity. A NanoPhotometer® spectrophotometer (IMPLEN, CA, USA) was used to check the purity of the RNA. Clustering and sequencing (Novogene Experimental Department) were conducted according to the manufacturer’s instructions. The index-coded samples were clustered using a cBot Cluster Generation System and TruSeq PE Cluster Kit v3-cBot-HS (Illumina). Finally, the libraries were sequenced using the Illumina NovaSeq platform and 150 bp paired-end reads were generated.
4
RNA-seq Analysis of Sorted Populations
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Total RNA was isolated from the four sorted populations using RNAiso Plus (Takara Bio). RNA-seq was performed at the Beijing Novogene Bioinformatics Technology Co, using the Illumina NovaSeq platform. RNA Nano 6000 Assay Kits and the Bioanalyzer 2100 system (Agilent Technologies, California, USA) were used to evaluate RNA integrity. A NanoPhotometer® spectrophotometer (IMPLEN, California, USA) was used to check the purity of the RNA. Clustering and sequencing (Novogene Experimental Department) were conducted according to the manufacturer's instructions. The index-coded samples were clustered using a cBot Cluster Generation System and TruSeq PE Cluster Kit v3-cBot-HS (Illumina). Finally, the libraries were sequenced using the Illumina NovaSeq platform and 150bp paired-end reads were generated.
5
Silkworm Fat Body Transcriptome Analysis
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Total RNA from the silkworm's fat body tissue at 2 and 6 h.p.i.20E was extracted using the Trizol reagent (Invitrogen) and further purified with RNeasy kits (Qiagen).
RNA purity was checked using a NanoPhotometer spectrophotometer (IMPLEN, CA, USA). RNA concentration was measured using Qubit RNA Assay Kits in a Qubit 2.0
Flurometer (Life Technologies, CA, USA). RNA integrity was assessed using RNA Nano6000 Assay Kits from the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
A total of 3 μg RNA per sample was used as input material for RNA sample preparation. Ribosomal RNA was removed using Epicentre Ribo-zero™ rRNA Removal Kits (Epicentre, USA), and rRNA-free residue was cleaned up by ethanol precipitation. Subsequently, sequencing libraries were generated using the rRNA-depleted RNA with NEBNext Ultra™ Directional RNA Library Prep Kits for Illumina (NEB, USA), following manufacturer's recommendations. Finally, the products were purified, and the library quality assessed using the Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kits v3-cBot-HS (Illumina), according to the manufacturer's instructions. After cluster generation, the libraries were sequenced on an Illumina HiSeq 2500 platform and 125 bp paired-end reads were generated.
RNA purity was checked using a NanoPhotometer spectrophotometer (IMPLEN, CA, USA). RNA concentration was measured using Qubit RNA Assay Kits in a Qubit 2.0
Flurometer (Life Technologies, CA, USA). RNA integrity was assessed using RNA Nano6000 Assay Kits from the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
A total of 3 μg RNA per sample was used as input material for RNA sample preparation. Ribosomal RNA was removed using Epicentre Ribo-zero™ rRNA Removal Kits (Epicentre, USA), and rRNA-free residue was cleaned up by ethanol precipitation. Subsequently, sequencing libraries were generated using the rRNA-depleted RNA with NEBNext Ultra™ Directional RNA Library Prep Kits for Illumina (NEB, USA), following manufacturer's recommendations. Finally, the products were purified, and the library quality assessed using the Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kits v3-cBot-HS (Illumina), according to the manufacturer's instructions. After cluster generation, the libraries were sequenced on an Illumina HiSeq 2500 platform and 125 bp paired-end reads were generated.
6
Transcriptome Profiling of Plant Samples
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Total RNA was extracted using the RNA prep Pure Plant Kit (Polysaccharides & Polyphenolics-rich), following the manufacturer’s protocol. RNA concentration was measured using the Qubit RNA Assay Kit in a Qubit 2.0 Flurometer (Life Technologies, CA, USA) and RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Following RNA quantification and qualification, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and the mRNA was then broken into fragments using a fragmentation buffer. First strand cDNA was synthesized using random hexamer primers, and second strand cDNA synthesis was subsequently performed using buffer, dNTPs, DNA Polymerase I, and RNase H. The library fragments were purified with AMPure XP beads (Beckman Coulter, Beverly, USA), and USER enzyme was used with size-selected, adaptor-ligated cDNA before PCR. PCR was performed to enrich the purified cDNA libraries. Finally, the library preparations were sequenced on an Illumina HiSeq platform.
7
RNA Extraction and Quality Evaluation
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Shoot samples (about 0.1 g containing leaf and stem) were collected 0 hour after cutting (HAC), 4 HAC, 2 DAC, and 8 DAC, frozen in liquid nitrogen and stored at -80°C. Total RNA was isolated using the improved CTAB method. RNA degradation and contamination was monitored on 1% agarose gels, RNA purity was checked using the Nano-Photometer® spectrophotometer (IMPLEN, Westlake Village, CA, USA), RNA concentration was measured using Qubit® RNA Assay Kit in Qubit®2.0 Fluorometer (Life Technologies, Camarillo, CA, USA) and RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA).
8
RNA-seq Library Preparation and Sequencing
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Total RNA was extracted from all three surgical specimens and the corresponding primary cells. The RNA concentration was measured using a Qubit® RNA Assay kit in a Qubit® 2.0 Fluorometer (both from Thermo Fisher Scientific, Inc.), and RNA integrity was assessed using the RNA Nano 6000 Assay kit with the Bioanalyzer 2100 system (both from Agilent Technologies, Inc.) according to the manufacturer's protocol. A total of 3 µg RNA per sample was used as the input material. Ribosomal RNA (rRNA) was removed using an Epicentre Ribo-zero™ rRNA Removal kit (Epicentre; Illumina, Inc.), according to the manufacturer's protocol. Subsequently, sequencing libraries were generated using rRNA-depleted RNA with the NEBNext® Ultra™ Directional RNA Library Prep kit for Illumina® (New England BioLabs, Inc.) according to the manufacturer's protocol. PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. Finally, the products were purified (AMPure XP system), and library quality was assessed on the Agilent Bioanalyzer 2100 system. Clustering of the index-coded samples was performed using a cBot Cluster Generation System with a TruSeq PE Cluster kit v3-cBot-HS (Illumina, Inc.). Following cluster generation, the libraries were sequenced on an Illumina Hiseq X-ten platform and 150-bp paired-end reads were generated (sequenced by Novogene Co., Ltd.).
9
Transcriptome Analysis with RNA Isolation
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The total RNA of each sample was isolated with TRIzol reagent (Ambion, Austin, TX, USA). A NanoPhotometer spectrophotometer (IMPLEN, Westlake Village, CA, USA) was used to check the purity of the RNA. The Qubit B RNA Assay Kit on a Qubit 2.0 fluorometer (Life Technologies, New York, NY, USA) was used to test the concentration of RNA. In addition, in order to evaluate RNA integrity, we used the RNA Nano 6000 Assay Kit in the Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). After passing the total RNA detection of the samples, the RNA libraries were prepared with a small RNA Sample Prep Kit (Illumina, San Diego, CA, USA).
10
RNA Extraction and Characterization
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Total RNA was extracted using TRIzol® Reagent (Invitrogen) according to the manufacturer’s protocol. The RNA was quantified by measuring the absorbance at 260 nm using a NanoVue UV-Vis spectrophotometer (Bio-Science). RNA purity was checked using a NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
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