Ab13970

Manufactured by Abcam

Sourced in United Kingdom, United States, Japan, Germany, France, Netherlands, Canada

Ab13970 is an antibody product manufactured by Abcam. The product is designed for use in laboratory research applications.

Automatically generated - may contain errors

Lab products found in correlation

A11039, by Thermo Fisher Scientific (35 mentions) Alexa fluor 488, by Thermo Fisher Scientific (25 mentions) Vectashield, by Vector Laboratories (17 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (13 mentions) Ab150169, by Abcam (12 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (12 mentions) Ab152, by Merck Group (11 mentions) Vt1000, by Leica camera (10 mentions) Alexa fluor 568, by Thermo Fisher Scientific (10 mentions) Mab377, by Merck Group (9 mentions) Lsm 880, by Zeiss (9 mentions) Alexa fluor 488 goat anti chicken igg, by Thermo Fisher Scientific (8 mentions) Lsm 700, by Zeiss (8 mentions) Lsm 780, by Zeiss (8 mentions) Ab290, by Abcam (7 mentions) Ab15580, by Abcam (7 mentions) Chicken anti gfp, by Abcam (7 mentions) Ab18465, by Abcam (7 mentions) Z0334, by Agilent Technologies (7 mentions) Ab5694, by Abcam (6 mentions)

504 protocols using ab13970

Antibody Validation for Insect Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used in this study:

Mouse anti-cockroach allatostatin (Ast7) (DSHB 5F10, 1:2 v/v dilution)

Mouse anti-fly BRP (DSHB nc82, RRID AB_23148662, 1:50 v/v dilution)

Mouse anti-fly Dlg (4F3, DSHB, RRIDAB_528203, 1:50 v/v dilution)

Chicken anti-GFP (Abcam ab13970, RRID AB_300798, 1:2000 v/v dilution)

Alexa 488 goat anti-chicken (A-11039 Thermo Fisher Scientific, RRID AB_2534096, 1:1000 v/v dilution)

Alexa 647 donkey anti-mouse (715-605-151, Jackson ImmunoResearch, RRID AB_2340863, 1:500 v/v dilution).

Alexa 568 goat anti-mouse (A-11004 Thermo Fisher Scientific, RRID: AB_2534072, 1:500 v/v dilution)

The validation of the four primary antibodies is as follows. The anti-allatostatin antibody (Ast7) (DSHB 5F10, 1:2 v/v dilution) was originally validated by immunohistochemistry^{96 (link)} and has since been documented in 12 publications. Mouse anti-fly disks large (DSHB 4F3) were raised against the second PDZ domain of Dlg^{95 (link)}, and have been widely used in Western blot, immunohistochemistry, and other applications in 95 publications. Chicken anti-GFP (Abcam ab13970, RRID AB_300798, 1:2000 v/v dilution) has been validated by Abcam via Western blot and immunohistochemistry. Mouse anti-fly BRP (DSHB nc82, RRID AB_23148662, 1:50 v/v dilution) was originally identified to bind to the Bruchpilot protein^{94 (link)} and has been documented in over 1000 publications.

Ott S., Xu S., Lee N., Hong I., Anns J., Suresh D.D., Zhang Z., Zhang X., Harion R., Ye W., Chandramouli V., Jesuthasan S., Saheki Y, & Claridge-Chang A. (2024). Kalium channelrhodopsins effectively inhibit neurons. Nature Communications, 15, 3480.

+ Open protocol

+ Expand

Immunofluorescence Analysis of Musculoskeletal Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols

Humeral head and supraspinatus tendon composite were harvested and fixed in the 4% paraformaldehyde in PBS overnight at room temperature. After decalcified and dehydrated, samples were embedded in Tissue-Tek O.C.T. Compound (SAKURA, Torrance, USA) and cut into 10 μm thickness of sagittal sections. Cell samples were fixed in the 4% paraformaldehyde in PBS for 30 min at room temperature. Both the parts and cell samples were blocked in 5% BSA for 40 min at room temperature and incubated with the primary antibodies anti-DMP1 (Abcam, 1:400, ab13970), anti-GFP (Abcam, 1:400, ab13970 or 1:400, ab290), anti-Sox9 (Abcam, 1:400; ab185966), anti-TGF-βR2 (Abcam, 1:400, ab186838) at 4 °C overnight. After washing, the sections were then incubated with the respective secondary antibodies (1:500, Abcam) for 1 hr at room temperature and sealed with DAPI. The images were captured with a Leica TCS-SP8 confocal microscope (Leica, Germany).

Xiao H., Zhang T., Li C., Cao Y., Wang L., Chen H., Li S., Guan C., Hu J., Chen D., Chen C, & Lu H. (2022). Mechanical stimulation promotes enthesis injury repair by mobilizing Prrx1+ cells via ciliary TGF-β signaling. eLife, 11, e73614.

+ Open protocol

+ Expand

Immunofluorescence Staining for Gene-Editing Effects

Check if the same lab product or an alternative is used in the 5 most similar protocols

In the immunofluorescence staining, the brain sections from monkey Middle age 2 were washed for 5 min in 0.01 mol/L PBS containing 5% bovine serum albumin (BSA) and 0.3% Triton X-100, and then incubated with a set of primary antibodies (in 0.01 mol/L PBS with 1% BSA and 0.3% Triton X-100) overnight at 4 °C.
In gene-editing effect tests, two sets of primary antibodies were used: (1) anti-EGFP (ab6673, Abcam), anti-NeuN (ABN78, Millipore), anti-Paris (75-195, NeuroMab); and (2) anti-EGFP (ab13970, Abcam), anti-NeuN (MABN377, Millipore), and anti-DJ-1 (ab201147, Abcam).
For glial cell co-staining, another set of primary antibodies was used: anti-GFAP (SMI-21R, Covance Research Products Inc.), anti-Iba1 (019-19741, Wako), and anti-EGFP (ab13970, Abcam).
All the sections were subsequently treated with the corresponding secondary antibodies (1:1000, Alexa-Fluor-conjugated, Invitrogen). DAPI was used to label nuclei and the sections were mounted with 75% glycerol for microscopy.

Li H., Wu S., Ma X., Li X., Cheng T., Chen Z., Wu J., Lv L., Li L., Xu L., Wang W., Hu Y., Jiang H., Yin Y., Qiu Z, & Hu X. (2021). Co-editing PINK1 and DJ-1 Genes Via Adeno-Associated Virus-Delivered CRISPR/Cas9 System in Adult Monkey Brain Elicits Classical Parkinsonian Phenotype. Neuroscience Bulletin, 37(9), 1271-1288.

+ Open protocol

+ Expand

Mapping Connections of PPG Neurons

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

YFP immunoperoxidase-labeled brainstem sections from 6 male and 2 female PPG-YFP mice were generated in previously published projects that focused on other research questions (Llewellyn-Smith et al., 2011 (link), 2013 (link)). Thus, while no new immunoperoxidase-labeled mouse material was generated for the present study, the cNTS-focused microscopic analysis of this material has not been previously reported. Close appositions by PPG-YFP axons onto PPG-YFP dendrites were identified in sections labeled using chicken anti-GFP (Abcam; 1:20K; AB_13970) and a Ni-intensified DAB reaction, as previously reported (Llewellyn-Smith et al., 2011 (link), 2013 (link)). Close appositions by PPG-YFP axons onto PPG-YFP cell bodie were identified in sections labeled using the same chicken anti-GFP antibody diluted 1:400K, followed by an imidazole-intensified DAB reaction.

Card J.P., Johnson A.L., Llewellyn‐Smith I.J., Zheng H., Anand R., Brierley D.I., Trapp S, & Rinaman L. (2018). GLP‐1 neurons form a local synaptic circuit within the rodent nucleus of the solitary tract. The Journal of Comparative Neurology, 526(14), 2149-2164.

+ Open protocol

+ Expand

Multicolor Immunohistochemistry of Transgenic Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

To allow adequate time for fluorophore expression, animals were perfused and sacrificed 2–4 weeks post-surgery. Transcardial perfusion was performed using saline, followed by 4% paraformaldehyde (PFA) in phosphate buffered saline (PBS). Dissected brains were post-fixed in 4% PFA overnight, followed by 30% sucrose solution in PBS for 2 days, or until brains sank. Brain sections (60 μm thickness) were first washed, blocked and stained with rabbit-anti-PV (1:1,000, Swant: PV-28, RRID:AB_2315235), chicken-anti-GFP (1:1,000, Abcam:AB_13970, RRID:AB_300798) and rat-anti-mcherry (1:1,000, mCherry Monoclonal Antibody (16D7) from Thermo Fisher Scientific, RRID:AB_2536611) primary antibodies overnight at RT, or for 2 days at 4°C. These sections were then washed and treated with secondary antibodies (1:1,000, Alexa Fluor 488, Alexa Fluor 568, and Alexa Fluor 647; RRID:AB_142924, RRID:AB_2534121, RRID:AB_2536101) at RT for 2–3 h. Stained sections were also stained with DAPI, to visualize cell nuclei.

Ham G.X, & Augustine G.J. (2022). Topologically Organized Networks in the Claustrum Reflect Functional Modularization. Frontiers in Neuroanatomy, 16, 901807.

+ Open protocol

+ Expand

Mapping Proglucagon Neuron Connectivity

Check if the same lab product or an alternative is used in the 5 most similar protocols

Yellow fluorescent protein immunoperoxidase‐labeled brainstem sections from six male and two female PPG‐YFP mice were generated in previously published projects that focused on other research questions (Llewellyn‐Smith et al., 2013; Llewellyn‐Smith, Reimann, Gribble, & Trapp, 2011). Thus, while no new immunoperoxidase‐labeled mouse material was generated for this study, the cNTS‐focused microscopic analysis of this material has not been previously reported. Close appositions by PPG‐YFP axons onto PPG‐YFP dendrites were identified in sections labeled using chicken anti‐GFP (Abcam; 1:20 K; AB_13970) and a Ni‐intensified DAB reaction, as previously reported (Llewellyn‐Smith et al., 2011, 2013). Close appositions by PPG‐YFP axons onto PPG‐YFP cell bodies were identified in sections labeled using the same chicken anti‐GFP antibody diluted 1:400 K, followed by an imidazole‐intensified DAB reaction.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Corneal Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunohistochemical staining was performed on experimental and control tissue sections as previously described (Singh et al., 2012 (link) and 2013 ). Briefly, sections from the central cornea were washed with PBS twice for five minutes each and permeabilized with 1% triton X-100 for one hour at room temperature. Sections were incubated with 4% normal goat serum with 1% BSA in PBS at room temperature for one hour to block non-specific binding. Sections were stained for α-smooth muscle actin with rabbit polyclonal antibody (ab5694, Abcam, San Francisco, CA) diluted 1:100 in 1% normal goat serum in PBS and/or for GFP with chicken polyclonal antibody (ab13970, Abcam) diluted 1:750 in 1% normal goat serum in PBS for 90 minutes. Sections were washed with PBS and then incubated with respective secondary antibodies (A11037 Invitrogen, Grand Island, NY) Alexa Fluor 594 goat anti-rabbit IgG, 1:200 dilution or A11039 (Invitrogen) Alexa Fluor 488 goat anti-chicken IgG, 1:1000 dilution, for 60 minutes before washing with PBS four times. Immunohistochemical controls were performed with omitted primary antibody. Coverslips were mounted with Vectashield containing DAPI (Vector Laboratories Inc., Burlingame, CA) to allow visualization of all nuclei in the tissue sections.

Singh V., Jaini R., Torricelli A.A., Santhiago M.R., Singh N., Ambati B.K, & Wilson S.E. (2014). TGFβ and PDGF-B signaling blockade inhibits myofibroblast development from both bone marrow-derived and keratocyte-derived precursor cells in vivo. Experimental eye research, 121, 35-40.

+ Open protocol

+ Expand

Immunocytochemistry Protocol for Cell Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunocytochemistry, cells were plated on glass cover slips coated with poly-d-lysine. Cells were washed 2× with ice cold 1× PBS, followed by fixation with fresh 4% paraformaldehyde for 15 min at room temperature (RT), blocked for 1 h at RT (10% goat serum and 0.3% Triton X-100 in 1× PBS), and incubated with primary antibodies (anti-HA, ab18181; anti-GFP, ab13970; anti-NeuN, ab104224; Abcam) at a concentration of 1:1000 at RT for 2 h or overnight at 4°C in 1:3 diluted blocking buffer. Slides were then washed and incubated with the appropriate Alexa Flour secondary antibodies (Abcam) at a concentration of 1:1000 for 1 h at RT. Slides were mounted using ProLong Gold Antifade Mountant with DAPI (Invitrogen) and images were acquired using a IX73 microscope (Olympus) and cellSens standard software.

Greer C.B., Wright J., Weiss J.D., Lazarenko R.M., Moran S.P., Zhu J., Chronister K.S., Jin A.Y., Kennedy A.J., Sweatt J.D, & Kaas G.A. (2021). Tet1 Isoforms Differentially Regulate Gene Expression, Synaptic Transmission, and Memory in the Mammalian Brain. The Journal of Neuroscience, 41(4), 578-593.

+ Open protocol

+ Expand

Immunostaining of Embryonic and Tissue Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunostaining, embryos and tissue were processed as previously described²⁴ and staining was performed on 100 µm thick vibratome sections in PBS with 0.3% Triton (PBST) and 3% BSA (Sigma A3608). Antisera were as follows: chicken anti-GFP 1/1000 (Abcam ab13970), rabbit anti-RFP 1/500 (Abcam ab62341), goat anti-Sox2 1/500 (R&D AF2018), rabbit anti-Sox9 (Millipore AB5535), rabbit anti-Prdm16 1/200 (gift from P. Seale⁶⁷), rabbit anti-Pax6 1/500 (Covance PRB-278P), rabbit anti-Hes1 1/100 (Cell Signalling D6P2U), goat anti-Gli3 1/500 (R&D AF3690). DNA was stained with DAPI. Fluorescent images were acquired using a Leica SP8 confocal microscope and analysed using ImageJ.

van den Ameele J., Krautz R., Cheetham S.W., Donovan A.P., Llorà-Batlle O., Yakob R, & Brand A.H. (2022). Reduced chromatin accessibility correlates with resistance to Notch activation. Nature Communications, 13, 2210.

+ Open protocol

+ Expand

Antibodies for Western Blotting and Immunostaining

Check if the same lab product or an alternative is used in the 5 most similar protocols

The antibodies used for western blotting (for human) were as follows: GFP (abcam, ab290, 1:2,000), α-tubulin (CST, 3873s, 1:2,000), GAPDH (CST, 2118s, 1:2,000), Flag (MBL, M185, 1:2,000), Myc (MBL, M047-3, 1:5,000), HA (MBL, M180-3, 1:5,000), Phospho-JNK (CST, 9255, 1:1,000), and WDR62 (bethyl, A310-550A, 1:1,000). For mouse, they were as follows: WDR62 (abcam, 1:1,000), WDR62 antibody generated by MBL company using antigen VGQGGNQPKAGPLRAGTC, Phospho-WDR62 1053T (present from Dominic) [52 (link)], and MEKK3 (CST, 5727, 1:1,000). The antibodies used for immunostaining were Sox2 (abcam, ab97959, 1:1,000), Pax6 (Covance, PRB-278P, 1:400), Tbr2 (Millipore, ab2283, 1:1,000), β-III Tubulin/Tuj1 (abcam, ab7751, 1:1,000), γ-Tubulin (abcam, ab11316, 1:1,000), α-Tubulin (CST, 3873, 1:2,000), Phosph-Histone 3 (P-H3) (abcam, ab10543, 1:1,000), Nestin (abcam, ab6142, 1:1,000), GFP (abcam, ab13970, 1:1,000), Ki67 (abcam, ab15580, 1:1,000), BrdU (abcam, ab6326, 1:500), Phospho-JNK (abcam, ab124956, 1:1,000), activated-caspase3 (abcam, ab13847, 1:1,000). Nuclei were stained with DAPI (4’,6-diamidino-2-phenylindole) (Invitrogen).

Xu D., Yao M., Wang Y., Yuan L., Hoeck J.D., Yu J., Liu L., Yeap Y.Y., Zhang W., Zhang F., Feng Y., Ma T., Wang Y., Ng D.C., Niu X., Su B., Behrens A, & Xu Z. (2018). MEKK3 coordinates with FBW7 to regulate WDR62 stability and neurogenesis. PLoS Biology, 16(12), e2006613.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!