Observer z1

Manufactured by Zeiss

Sourced in Germany, United States, United Kingdom, France

The Observer Z1 is a versatile microscope system designed for laboratory and research applications. It offers high-quality optical performance and advanced imaging capabilities. The core function of the Observer Z1 is to provide clear and detailed observation of a wide range of samples, enabling users to conduct detailed analysis and study various specimens.

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Lab products found in correlation

Axiocam mrm camera, by Zeiss (14 mentions) Lsm 710, by Zeiss (14 mentions) Axiovision software, by Zeiss (10 mentions) Axiovision 4, by Zeiss (10 mentions) Axiocam mrm, by Zeiss (10 mentions) Lsm 700, by Zeiss (8 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (7 mentions) Zen blue software, by Zeiss (6 mentions) Plan apochromat 20x 0.8 m27 objective, by Zeiss (6 mentions) Axio imager, by Zeiss (6 mentions) Axiocam mrc, by Zeiss (6 mentions) Lsm 880, by Zeiss (6 mentions) Ultraview vox, by PerkinElmer (5 mentions) Metamorph 7, by Molecular Devices (5 mentions) Triton x 100, by Merck Group (5 mentions) Zen software, by Zeiss (5 mentions) Bioscope catalyst, by Bruker (4 mentions) Axiocam hrm camera, by Yokogawa (4 mentions) Lsm 780, by Zeiss (4 mentions) Inverted microscope, by Zeiss (4 mentions)

439 protocols using observer z1

Cell Viability and Degradation in Fibrin Hydrogels

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To analyze cell viability in the HS fibrin gels, passage 3–5 AFC were dissociated and resuspended in EGM-2 (Lonza) at 4x10⁵ cells/mL. 75 uL HS (250 mM NaCl) and PS (145 mM NaCl) fibrin gels were fabricated using the 4X AFC suspension as drops in wells of a 6-well tissue culture-treated plate (three gels per well). After gelation, 2 mL of EGM-2 was added to each well and the gels were incubated at 37°C and 5% CO₂. After 1, 24, and 96 hours, EGM-2 was aspirated and cells were stained using the fluorescent LIVE/DEAD Viability/Cytotoxicity Kit (Invitrogen) according to kit instructions. Three images were captured from each gel using a Zeiss Observer.Z1 and long-distance objective (LD Plan-NEOFLUAR 20X/0,4 Ph2) and were used to count living and dead cells.
To analyze the cell-mediated degradation kinetics of HS and PS fibrin formulations, gels were fabricated with a final concentration of 1x10⁵ AFC/mL (P3-5) and incubated in EGM-2 +/- 1 mg/mL of the plasmin inhibitor 6-aminocaproic acid (Sigma, A2504) at 37°C and 5% CO₂. After 0, 7, and 14 days, gels were imaged using phase contrast (Zeiss ObserverZ.1) and wet weights were recorded.

Jarrell D.K., Vanderslice E.J., Lennon M.L., Lyons A.C., VeDepo M.C, & Jacot J.G. (2021). Increasing salinity of fibrinogen solvent generates stable fibrin hydrogels for cell delivery or tissue engineering. PLoS ONE, 16(5), e0239242.

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Brightfield and Confocal Imaging of Zebrafish Tissues

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Brightfield images for histological and ISH sections were captured using Axiovision camera ICc1 mounted on the Zeiss observer Z1 (10X 0.3 NA; 20X 0.8 NA). Confocal images of whole mount immunofluorescence staining and FISH were collected using Zeiss observer Z1 (40X 1.1 NA W). All zebrafish were deyolked and imaged from the ventral side. Z-sections analyzed always included the optic nerve for consistency. Confocal imaging allowed us to collect images that were not saturating the photomultiplier tube detector, and thus linear fluorescence intensity is linear within the entire dynamic range.

Muralidharan P., Sarmah S, & Marrs J.A. (2018). Retinal Wnt signaling defect in a zebrafish fetal alcohol spectrum disorder model. PLoS ONE, 13(8), e0201659.

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Boyden Chamber Assay for Cell Migration

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To measure cell migration, an adapted Boyden chamber assay was used as previously described [26 (link)]. To this end, cells were incubated for 20 h in serum-free DMEM and then seeded into a transwell filter (6.5 μm diameter, 8 μm pore size) at a density of 5 × 10⁴ cells in 200 μL starvation medium. Then, 600 μL of DMEM medium containing 1% FBS was filled in the lower compartment, and cells were allowed to migrate for 20 h at 37 °C. Migrated cells in the lower chamber were counted in five different random fields per well using a bright field microscope (Zeiss Observer Z1). Alternatively, MCF-7 cells that had migrated into the filter were stained with 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI) (1 μg/mL in methanol) and quantified in five random fields under a fluorescent microscope (Zeiss Observer Z1).

Schwalm S., Erhardt M., Römer I., Pfeilschifter J., Zangemeister-Wittke U, & Huwiler A. (2020). Ceramide Kinase Is Upregulated in Metastatic Breast Cancer Cells and Contributes to Migration and Invasion by Activation of PI 3-Kinase and Akt. International Journal of Molecular Sciences, 21(4), 1396.

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Rapid Mycobacterial Detection via Auramine Staining and DMN-Tre Labeling

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Auramine smear was performed according to standard protocol included in the kit (Fluorescent Stain Kit for mycobacteria, 05151, Sigma- Aldrich). Briefly, one-half of NalC/NaOH-decontaminated sputum sample was smeared onto microscopy slides and heat-fixed (heating block, 95°C; 5 to 10 min). Smears were then treated with Auramine O for 5 min, destained, and then counterstained before viewing in the FITC/GFP channel using a Zeiss Observer Z1-inverted fluorescence microscope.
For DMN-Tre labeling, the other half of the same sample was stained as follows: 100 μl of sample was incubated with 1 mM DMN-Tre for 30 min at 37°C, followed by fixing in 2.5% glutaraldehyde for 1.5 hours. Samples were then resuspended in 30 μl of 1× PBS. Twenty microliters was mounted on a 2% agarose pad for viewing in the FITC and differential interference contrast channels of a Zeiss Observer Z1-inverted fluorescence microscope.

Kamariza M., Shieh P., Ealand C.S., Peters J.S., Chu B., Rodriguez-Rivera F.P., Babu Sait M.R., Treuren W.V., Martinson N., Kalscheuer R., Kana B.D, & Bertozzi C.R. (2018). Rapid detection of Mycobacterium tuberculosis in sputum with a solvatochromic trehalose probe. Science translational medicine, 10(430), eaam6310.

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Eyelid Morphology and Meibum Production

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The heads of PD0–PD7 pups and eyes of PD21 and PD50 mice were fixed in 4% paraformaldehyde in 0.1 M phosphate buffer at 4 °C overnight. The eyelids were dissected, washed extensively in PBS, and subjected to subsequent evaluation.
For morphological evaluation, the eyelids were first incubated in permeablization/staining buffer (0.25% DMSO, 0.25% Triton-100 and 15% sucrose in PBS) for 30 min at room temperature, then stained with Alexa-546 Phalloidin (1:20) and DAPI in permeablization/staining buffer at 4 °C overnight. The eyelids were washed with permeablization/staining buffer twice, transferred to PBS, mounted on glass slides in 50% glycerol and photographed under a fluorescent microscope (Zeiss Observer. Z1).
For functional evaluation of meibum production, the eyelids derived from PD7 pups or older mice were rinsed with 60% Isopropanol for 5 min, followed by incubation with ORO (ORO 300 mg/ml) for 30 min at room temperature. After washing with 60% Isopropanol twice for 3 min each time and rinsing with PBS, the samples were subjected to staining with Alexa-488 Phalloidin and DAPI at 4 °C overnight. The samples were transferred to PBS, mounted on glass slides in 50% glycerol and photographed under a fluorescent microscope (Zeiss Observer. Z1).

Wang J., Call M., Mongan M., Kao W.W, & Xia Y. (2017). Meibomian gland morphogenesis requires developmental eyelid closure and lid fusion,. The ocular surface, 15(4), 704-712.

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Bright and Fluorescent Microscopy Imaging

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Bright field images were acquired on an IX8I (Olympus) coupled to a digital camera (U-TV 0.5XC; Olympus) using the AnalySIS software (Soft Imaging System, GmbH, Southend-on-Sea, UK) or on an Observer Z1 (Zeiss, Cambridge, UK) microscope coupled to an EMCCD (Photometrics PVCam, Marlow, UK) camera using the Axiovision software (Carl Zeiss Inc., Cambridge, UK). The objectives used were UPlanFI 4 × NA 0.13, LCPlanFI 20 × NA 0.40 (all air objectives) and Zeiss EC Plan Neoflur 20 × 0.5NA objectives. Fluorescent images were acquired using Observer Z1 (Zeiss) microscope coupled to an EMCCD (Photometrics PVCam) camera using the Axiovision software (Carl Zeiss Inc.). The fluorophores used were GFP and DsRed2. Images were acquired at 37 °C or room temperature.

Di Stefano M., Nascimento-Ferreira I., Orsomando G., Mori V., Gilley J., Brown R., Janeckova L., Vargas M.E., Worrell L.A., Loreto A., Tickle J., Patrick J., Webster J.R., Marangoni M., Carpi F.M., Pucciarelli S., Rossi F., Meng W., Sagasti A., Ribchester R.R., Magni G., Coleman M.P, & Conforti L. (2014). A rise in NAD precursor nicotinamide mononucleotide (NMN) after injury promotes axon degeneration. Cell Death and Differentiation, 22(5), 731-742.

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Filamentous Microorganism Quantification in MBR Activated Sludge

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Filamentous Index (FI) was used, according to the Eikelboom method [27 ,28 ], to measure the population of filamentous microorganisms in the activated sludge mixed liquor of the MBR units. For the FI measurement, a Light Sheet Microscope (LSM, Observer Z1, Zeiss, Oberkochen, Germany) was used mainly in 100× magnification, whereas in some cases 50× and 200× magnifications were, also, used to take a better view of the samples. In filamentous index measurement, FI = 0 corresponds to no filaments coming out of the sludge flocs, whereas FI = 5 corresponds to infinite filaments. ZEN software for microscope and imaging was used to edit the images, creating tiff image files. FI was measured for mixed liquor samples from all the tanks of both MBR units and they were found similar for the tanks of each MBR unit, as both MBRs were in balance. Gram staining and Neisser staining procedures [28 ] were, also, performed using the Light Sheet Microscope (LSM, Observer Z1, Zeiss) in 200× magnification to identify filamentous microorganisms.

Banti D.C., Mitrakas M, & Samaras P. (2021). Membrane Fouling Controlled by Adjustment of Biological Treatment Parameters in Step-Aerating MBR. Membranes, 11(8), 553.

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Evaluating Yak Cumulus Cell Proliferation and Apoptosis

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The proliferation of yak CCs was evaluated with the EdU assay as described in our previous study [54 (link)]. In brief, cultured yak CCs (2 × 10³ cells/well) were washed with PBS three times, and 50 mM EdU (RiboBio, Guangzhou, China) was supplemented and co-incubated for 3 h at a final concentration of 50 mmol/L. Subsequently, these nuclei were stained with 5 g/L DAPI for 5 min. Finally, the cells were counted, and photos were obtained under the same intensity of fluorescence using a fluorescence microscope (Observer Z1, Zeiss, Oberkochen, Germany).
The apoptotic rate of yak CCs was detected with the TUNEL detection kit (Yeasen, Shanghai, China) according to the manufacturer’s instructions. Specifically, after being fixed with 4% paraformaldehyde for 4 h, yak CCs were incubated with DNase I (10 U/mL) for 10 min, and CCs were treated with dATP and TdT for 10 min at RT. These cellular nuclei were stained with 5 g/L DAPI. Finally, photos were obtained under the same intensity of fluorescence using a fluorescence microscope (Observer Z1, Zeiss, Oberkochen, Germany).

Xiong X., Hu Y., Pan B., Zhu Y., Fei X., Yang Q., Xie Y., Xiong Y., Lan D., Fu W, & Li J. (2023). RFRP-3 Influences Apoptosis and Steroidogenesis of Yak Cumulus Cells and Compromises Oocyte Meiotic Maturation and Subsequent Developmental Competence. International Journal of Molecular Sciences, 24(8), 7000.

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Fluorescence and Electron Microscopy of Bt-treated Worms

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For fluorescence microscopy elt-2(RNAi) worms were exposed to Bt in 6 cm diameter plates and at each time point 10 worms were transferred to a microscopic slide with an agar pad, immobilized with 50 mM sodium azide and observed with a Carl Zeiss Observer Z.1 inverted fluorescence microscope or the confocal Carl Zeiss LSM 700. For light microscopy, samples were observed under the Carl Zeiss Observer Z.1 microscope. For TEM, worms were exposed to Bt as described for RNA extractions and washed off plates 12 h p.i. directly with fixation solution (2.5% glutaraldehyde, 2% paraformaldehyde, 0,1 M cacodylate buffer pH 7.2). Worms were cut in half to ensure penetration of the fixation solution and prepared as described in [93 ] for visualization with a Tecnai 2 electron microscope in the microscopy facility of the Biology Tower at the Christian-Albrechts University Kiel.

Zárate-Potes A., Yang W., Pees B., Schalkowski R., Segler P., Andresen B., Haase D., Nakad R., Rosenstiel P., Tetreau G., Colletier J.P., Schulenburg H, & Dierking K. (2020). The C. elegans GATA transcription factor elt-2 mediates distinct transcriptional responses and opposite infection outcomes towards different Bacillus thuringiensis strains. PLoS Pathogens, 16(9), e1008826.

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Immunofluorescence Staining of Cultured Cells

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Immunofluorescence staining for cultured cells was performed on μ-slide 8 well (ibidi). Cells were fixed in 4% paraformaldehyde for 15 min at room temperature, washed twice in Tris-buffered saline with 0.05% tween 20 (TBS-T) and blocked 60 min with blocking solution (TBS-T containing 1% bovine serum albumin and 1% Block Ace). The fixed cells were immersed in a permeabilizing solution (0.2% Triton-X100/TBS-T) for 10 min. Primary antibodies, Foxa2, Sox2, and Foxn1, were prepared as 1:200 dilution with antibody diluent (1%BSA/TBS-T) and added overnight at 4 °C (Supplementary table 2). Cells were washed and incubated with donkey anti-rabbit IgG-AlexaFlour555, goat anti-mouse IgG-AlexaFlour488, and donkey anti-goat IgG-AlexaFlour555 for 1 h at 4 °C. Nuclei were stained with DAPI. Stained samples were visualized using fluorescent microscopy (Zeiss Observer Z1, Zeiss) and images were captured and analyzed by AxioVision software (Zeiss).

Otsuka R., Wada H., Tsuji H., Sasaki A., Murata T., Itoh M., Baghdadi M, & Seino K.I. (2020). Efficient generation of thymic epithelium from induced pluripotent stem cells that prolongs allograft survival. Scientific Reports, 10, 224.

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