The activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum samples were measured using the Sigma ALT and AST Activity Assay Kit (St. Louis, MO) and read using the BioTek Synergy H1 Hybrid Multi-Mode Reader plate reader (BioTek, Winooski, VT). Serum triglyceride content was measured using the Cayman Chemical Triglyceride Colorimetric Assay kit (Cayman Chemical, Ann Arbor, MI). Serum BDNF was measured using the R&D Systems (Minneapolis, MN, USA) Total BDNF Quantikine ELISA Kit and following the kit protocol and guidelines for serum tissue analysis.
Triglyceride colorimetric assay kit
Manufactured by Cayman Chemical
Sourced in United States, Germany
The Triglyceride Colorimetric Assay Kit is a laboratory tool designed to quantify the levels of triglycerides in a given sample. The kit utilizes a colorimetric reaction to measure the triglyceride concentration, providing a reliable and accurate method for analysis.
Automatically generated - may contain errors
Lab products found in correlation
Cholesterol fluorometric assay kit, by Cayman Chemical (11 mentions)
Ultra sensitive mouse insulin elisa kit, by Crystal Chem (11 mentions)
Cholesterol quantification kit, by Abcam (6 mentions)
Mouse ultrasensitive insulin elisa kit, by ALPCO (6 mentions)
Bca protein assay, by Thermo Fisher Scientific (6 mentions)
Reflotron got, by Roche (4 mentions)
Reflotron gpt, by Roche (4 mentions)
Envision multilabel plate reader, by PerkinElmer (4 mentions)
Cholesterol quantitation kit, by Merck Group (4 mentions)
Free fatty acid fluorometric assay kit, by Cayman Chemical (4 mentions)
Free fatty acid quantification kit, by Abcam (4 mentions)
Glucose colorimetric assay kit, by Cayman Chemical (4 mentions)
Glycogen assay kit, by Merck Group (4 mentions)
Cholesterol assay kit, by Abcam (4 mentions)
Np40 substitute assay reagent, by Cayman Chemical (4 mentions)
Pierce bca protein assay kit, by Thermo Fisher Scientific (4 mentions)
Standard diluent assay reagent, by Cayman Chemical (3 mentions)
Reflovet plus, by Roche (3 mentions)
Oil red o staining kit, by Lifeline Cell Technology (3 mentions)
Insulin, by Merck Group (3 mentions)
266 protocols using triglyceride colorimetric assay kit
1
Serum Biomarker Analysis Protocol
2
Serum Biomarker Quantification Protocol
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The activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum samples were measured using the Sigma ALT and AST Activity Assay Kit (St. Louis, MO) and read using the BioTek Synergy H1 Hybrid Multi‐Mode Reader plate reader (BioTek, Winooski, VT). Serum triglyceride content was measured using the Cayman Chemical Triglyceride Colorimetric Assay Kit (Cayman Chemical, Ann Arbor, MI). Serum BDNF was measured using the R&D Systems (Minneapolis, MN, USA) Total BDNF Quantikine ELISA Kit and following the kit protocol and guidelines for serum tissue analysis.
3
Quantifying Lipid Accumulation in 3T3-L1 Adipocytes
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3T3-L1 pre-adipocytes were cultured and fully differentiated with and without BME
during 6 days. The cells were washed twice with PBS and fixed with 10% formalin
for 30 min. After fixation, they were washed with 60% isopropanol for 5 min and
then stained with oil-red O working solution (1.5 mg/mL oil-red O/60%
isopropanol) for 15 min at RT. Then, they were washed with distilled water and
photographed under a light microscope. Next, the TG levels were measured in cell
lysates using Triglyceride Colorimetric Assay Kit (Cayman, Ann Arbor, MI, USA).
Cells were washed twice with PBS then scraped and centrifuged at 100×g
for 5 min. Cell pellets were lysed in standard diluent buffer and centrifuged at
10,000×g for 10 min. The supernatant was collected and measured the
TG.
during 6 days. The cells were washed twice with PBS and fixed with 10% formalin
for 30 min. After fixation, they were washed with 60% isopropanol for 5 min and
then stained with oil-red O working solution (1.5 mg/mL oil-red O/60%
isopropanol) for 15 min at RT. Then, they were washed with distilled water and
photographed under a light microscope. Next, the TG levels were measured in cell
lysates using Triglyceride Colorimetric Assay Kit (Cayman, Ann Arbor, MI, USA).
Cells were washed twice with PBS then scraped and centrifuged at 100×g
for 5 min. Cell pellets were lysed in standard diluent buffer and centrifuged at
10,000×g for 10 min. The supernatant was collected and measured the
TG.
4
Triglyceride Quantification in Tissue Samples
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Tissue samples were homogenised in the Standard Diluent Assay Reagent provided by a Triglyceride Colorimetric Assay kit (Cayman Chemical, Ann Arbor, MI) and triglyceride levels were quantified based on reference standards as described in the manufacturer's instructions.19
5
Quantification of Plasma and Tissue Lipids
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Plasma glucose level was measured by a commercial kit of LabAssayTM Glucose (Fujifilm, Wako Pure Chemical Corporation, Osaka, Japan). Plasma triglycerides (TG) levels were measured by a commercial kit of LabAssayTM Triglycerides (Fujifilm, Wako Pure Chemical Corporation, Osaka, Japan). Intestinal and hepatic triglycerides (TG) contents were measured by the Triglyceride Colorimetric Assay Kit (Cayman Chemical, Ann Arbor, MI, USA). The protein concentrations in the intestine and liver were detected by Bradford method (Beyotime, P0006, Shanghai, China) to normalize the content of tissue TG. All of the measurements were performed following the manufacturer’s instructions.
6
Triglyceride Quantification in Muscle
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The triglyceride content in muscle homogenates was measured using the triglyceride colorimetric assay kit (Cayman, 10,010,303) by following the manufacturer’s protocol. In a 96 well plate, a standard curve was prepared using the included standard reagents and diluents. In each well, 10 µL of sample and 150 µL of the assay enzyme solution were added, thoroughly mixed on a microplate shaker (FisherBrand, 88,861,023), and then incubated for 30 min at 37°C. The absorbance of theassay was measured using a TECAN plate reader (TECAN, Infinite m200) and Magellan software. Absorbance of samples was analyzed to determine differences between WT and KO groups.
7
Triglyceride Quantification Protocol
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Triglyceride (TAG) levels were measured with the Triglyceride Colorimetric Assay Kit (#10010303; Cayman Chemical, Ann Arbor, MI). Samples were run according to the manufacturer's protocol in triplicate. TAG concentrations were normalized relative to protein concentration using the BCA protein assay (Pierce Biotechnology).
8
Faecal Lipid Extraction and Analysis
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Faeces were collected and dried in an oven at Week 6. Subsequently, 0.1 g of faeces were pulverised in 1 mL of phosphate buffered saline and extracted using a solvent (chloroform:methanol = 2:1). The organic phase was filtered using filter paper (Whatman NO.5). Afterwards, the residue was dried and reconstituted with 1 mL of dimethyl sulphoxide. Water bath sonication was used to dissolve the precipitate. A cholesterol liquid assay (Randox, Antrim, UK), Triglyceride Colorimetric Assay Kit (Cayman, Ann Arbor, MI, USA) and Total Bile Acids Assay Kit (Crystal Chem, Downers Grove, IL, USA) were adopted to analyse faecal cholesterol, triglyceride and bile acid contents.
9
Quantifying Serum Lipid Levels
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Serum levels of free fatty acids, glycerol, total cholesterol, and triglycerides were measured using free fatty acid quantification kits (ab65341, Abcam, Cambridge, UK), a glycerol colorimetric assay kit (10010755, Cayman Chemical, Ann Arbor, MI, USA), a total cholesterol colorimetric assay kit (MBS2540484, Mybiosource, San Diego, USA), and a triglyceride colorimetric assay kit (10010303, Cayman Chemical), respectively.
10
Triglyceride Quantification in Liver Tissue
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Whole maternal livers were pulverized in liquid nitrogen. Thereafter, 50 mg of the pulverized tissue was used for triglyceride quantification using the Triglyceride Colorimetric Assay Kit (Cayman) according to the manufacturer’s instructions. Whole fetal livers were used for triglyceride concentration quantification using the same method.
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