Silk from Bombyx mori was sourced from the cocoons of sericulture silkworms maintained in the facilities of IMIDA (Murcia, Spain) and fed on fresh Morus alba L. leaves. The cocoons were treated to remove the sericin by boiling in an aqueous solution of Na2CO3 (0.05 N) twice for 60 min. After washing with pure water and air-drying, the resulting substance had a bright white, cotton-like appearance. The silk was dissolved in the ionic liquid 1-ethyl-3-methylimidazolium acetate [emim+][acetate−] by high-power ultrasound, as described in a previous report [12 (link)]. The ionic liquid (97% purity) was obtained from Io-LiTec GmbH (Frankfurt, Germany) and was applied without any further purification. Ibrutinib (IB) [38 (link)] (Figure S1 of Supporting Information ) was provided by AdooQ Bioscience (Irvine, CA, USA). Highly purified water (18.2 MΩ·cm at 25 °C; from a Millipore Direct-Q1 ultrapure water system, Billerica, MA, USA) was used throughout. N-Hydroxysuccinimide (NHS) and N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) were purchased from Merck. All other chemicals and solvents were of analytical grade and were used without any extra purification.
N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride
Manufactured by Merck Group
Sourced in United States, Germany, United Kingdom, France, Canada, China, Italy, Poland, Sao Tome and Principe, Macao
N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride is a water-soluble carbodiimide compound commonly used as a coupling agent in organic synthesis and biochemistry. It facilitates the formation of amide bonds between carboxyl and amino groups.
Automatically generated - may contain errors
Lab products found in correlation
N hydroxysuccinimide, by Merck Group (122 mentions)
Bovine serum albumin (bsa), by Merck Group (32 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (30 mentions)
Hydrochloric acid (hcl), by Merck Group (21 mentions)
Sodium hydroxide (naoh), by Merck Group (18 mentions)
Phosphate buffered saline (pbs), by Merck Group (17 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (15 mentions)
N hydroxysuccinimide nhs, by Merck Group (13 mentions)
Sodium chloride (nacl), by Merck Group (13 mentions)
Ethanol, by Merck Group (12 mentions)
N hydroxysulfosuccinimide sodium salt, by Merck Group (12 mentions)
Citric acid, by Merck Group (12 mentions)
Methanol, by Merck Group (11 mentions)
4 dimethylaminopyridine, by Merck Group (10 mentions)
Acetic acid, by Merck Group (9 mentions)
Acetone, by Merck Group (9 mentions)
Ascorbic acid, by Merck Group (8 mentions)
Dopamine hydrochloride, by Merck Group (8 mentions)
Eah sepharose 4b resins, by GE Healthcare (8 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (8 mentions)
270 protocols using n 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride
1
Silk Extraction and Ionization Protocol
2
Brucella Immunodetection and SPR Analysis
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Brucella Selective Broth (BSB) and Agar (Hi-media), Polyclonal goat and rabbit anti-mice immunoglobulins/HRP, Polyclonal goat anti-rabbit immunoglobulins/HRP (Dako-Denmark), O-Phenylenediamine dihydrochloride (OPD) from Sigma-Aldrich, 3,3’-Diaminobenzidine (DAB) Sigma-Fast Tablets, Protein-A antibody purification kit (Montage-Millipore, USA). Pure analytical grade chemicals for SPR work from Sigma-Aldrich (Fluka) and Merck as; N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), 4-Mercaptobenzoic acid (4-MBA), Ethanolamine, Heparin (Hi-media), Sodium Azide, Methanol (MeOH), Phosphate Buffered Saline (PBS, pH 7.2, 10 mM L−1) and Hydrochloric acid (0.01 M HCl).
3
Gelatin-Chitosan Biomaterial Synthesis
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Gelatin type B and chitosan of medium weight were purchased from Sigma-Aldrich (USA). N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC), trifluoroacetic acid (TFA), dichloromethane (DCM) and ethanol were purchased from Merck (Germany). DMEM/F12 medium, FBS, trypsin/EDTA, l -glutamine, and penicillin/streptomycin were purchased from Gibco, Canada.
4
Synthesis and Characterization of Curcumin-Loaded Nanoparticles
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PEI, branched) MW = 1800, purity ≥99%, CAS number: 9002-98-6), was obtained from Alfa Aesar (Heysham, United Kingdom). N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC, CAS number: 25952-53-8), N-hydroxysuccinimide (NHS, CAS number: 6066-82-6), dichloromethane (DCM, CAS number: 75-09-2), and dimethyl sulfoxide (DMSO, CAS number: 67-68-5) were purchased from Merck (Kenilworth, USA). OL (purity ≥99%, CAS number: 112-80-1), thiazolyl blue tetrazolium bromide (MTT, CAS number: 57360-69-7), and curcumin were acquired from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco's modified Eagle's medium (DMEM) was obtained from INOCLON (Tehran, Iran). Fetal bovine serum (FBS, Product number: F7524), penicillin–streptomycin (pen-strep, Product number: P0781), and BSA (CAS number: 9048-46-8) were acquired from Sigma-Aldrich (St. Louis, MO, USA). RAW264.7 cell lines were purchased from the Iranian Biological Resource Center (Tehran, Iran, IBRCC 10072).
5
Conjugation of Dyes and PEGs to Latex Beads
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Latex-NH2 beads (φ = 25 μm) were purchased from micromod Partikeltechnologie GmbH (Rostock, Germany). N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) was purchased from Merck KGaA (Darmstadt, Germany). Dimethyl sulfoxide (DMSO), urea, phosphate buffered saline (PBS), and piperidine were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). 3-amino-3-(2-nitrophenyl) propionic acid (ANP) was purchased from Alfa Aesar (Haverhill, MO, USA). Cy5-NH2 was purchased from Lumiprobe Corporation (Hunt Valley, MD, USA). Fluorescein (FITC)-PEG (2k, 5k, 10k)-NHS and mPEG (2k, 5k, 10k)-NHS were purchased from Funakoshi CO., Ltd. (Tokyo, Japan). FITC labeled albumin was purchased from ThermoFisher Scientific (Waltham, MA, USA).
6
Immunohistochemical Localization of LTPs and ABA
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The roots were fixed for studying the immunohistochemical localization of LTPs and ABA at one and seven days after the introduction of sodium chloride into the root environment. Therefore, pieces of root tissue were taken from its central (middle part) and basal parts. Five millimeter root segments were fixed for 12 h in a solution of 4% N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (Merck, Darmstadt, Germany) prepared in 0.1 M phosphate buffer (pH 7.2–7.4). Carbodiimide not only fixes proteins, but also conjugates ABA with tissue proteins [16 ,17 (link)]. Then, root tissues were placed in a mixture of 4% paraformaldehyde (Riedel de Haen, Seelze, Germany) and 0.1% glutaraldehyde (Sigma) for 12 h. After fixation, plant tissues were washed for 1 h in phosphate buffer and successively kept for 30 min in ethanol dilutions for their dehydration. Pieces of roots were embedded in JB4 resin (Electron Microscopy Sciences, Hatfield, PA, USA). Histological sections 1.5 μm thick were obtained using a rotary microtome (HM 325, MICROM Laborgerate, Germany).
7
Gold Nanoparticle Synthesis and Functionalization
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Gold(III) chloride trihydrate (HAuCl4·3H2O), trisodium citrate dihydrate (C6H5O7Na3·2H2O), L-(+)-ascorbic acid (AA), silver nitrate (AgNO3), hydrochloric acid (HCl), nitric acid (HNO3—70%), Poly(ethylene glycol) 2-mercaptoethyl acetic acid (SH-PEG-COOH, Mn 7500), N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC), N-Hydroxysuccinimide (NHS), 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate (HATU), trifluoroacetic acid (TFA), triphenylmethanol, triisopropylsilane (TIPS), 2-thioethylamine, benzoic acids, and all organic solvents were purchased from Merck. Biotinylated antibody, biotinylated albumin serum bovine (BSA), streptavidin, and Alexa550-WGA were purchased from ThermoFisher Scientific. DMEM/F12 medium, FBS, penicillin/streptomycin solution, and Opti-MEM were purchased from ThermoFisher Scientific.
8
Functionalized SWNTs for Water Analysis
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N-Hydroxysulfosuccinimide sodium salt (NHS), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), deferoxamine mesylate salt (DFO), MES hydrate buffer, iron standard solution, and aluminium standard solution were purchased from Aldrich and used as received without further purification. Calcium chloride dihydrate and nickelous chloride 6-hydrate were purchased from J. T. Baker and used as received. Zinc chloride, copper reference standard solution, cadmium reference standard solution, and lead reference standard solution were purchased from Fisher and used as received. Magnesium chloride hexahydrate was purchased from EM science Merck kGaA and used as received. Rain water samples were collected from Newark in New Jersey with sampling details in the previous work.64 The sample was diluted 106 times before it was used in this experiment. Highly purified single-walled carbon nanotubes (SWNTs) were prepared via the HiPCO process and obtained from Carbon Nanotechnologies. 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N[carboxy(polyethylene glycol)-2000] (ammonium salt) (PL–PEG–COOH) was purchased from Avanti Polar Lipids Inc. All solutions were prepared using deionized water (18.2 M) (Nanopore water, Barnstead).
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N-Hydroxysulfosuccinimide sodium salt (NHS), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), deferoxamine mesylate salt (DFO), MES hydrate buffer, iron standard solution, and aluminium standard solution were purchased from Aldrich and used as received without further purification. Calcium chloride dihydrate and nickelous chloride 6-hydrate were purchased from J. T. Baker and used as received. Zinc chloride, copper reference standard solution, cadmium reference standard solution, and lead reference standard solution were purchased from Fisher and used as received. Magnesium chloride hexahydrate was purchased from EM science Merck kGaA and used as received. Rain water samples were collected from Newark in New Jersey with sampling details in the previous work.64 The sample was diluted 106 times before it was used in this experiment. Highly purified single-walled carbon nanotubes (SWNTs) were prepared via the HiPCO process and obtained from Carbon Nanotechnologies. 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N[carboxy(polyethylene glycol)-2000] (ammonium salt) (PL–PEG–COOH) was purchased from Avanti Polar Lipids Inc. All solutions were prepared using deionized water (18.2 M) (Nanopore water, Barnstead).
9
Synthesis and Characterization of Functionalized Boltorn H40
10
Polymer-Peptide Conjugate Synthesis
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