Inveon pet ct multimodality system

The imaging study was started when the tumor size reached 200–500 mm³ on a Inveon PET/CT Multimodality System (Siemens Medical Solutions USA, Inc.,Knoxville, TN, USA). Followed by CT data acquisition, which was conducted at 80 kV and 500 μA with a focal spot of 58 μm, a dynamic scan (0–60 min) was performed immediately after intravenous injection of ~3.5 MBq of ¹⁸F-SynVesT-1 in 100 μL saline containing < 5% ethanol into each tumor-bearing mouse under anesthesia, with 2% isoflurane in oxygen. After the dynamic scan, the mouse was allowed to recover in a cage and then re-anesthetized for a 20 min scan at 2.5 and 4 h post-injection (p.i.) (at each time point, n = 3–4). Both CT and PET images were reconstructed with the manufacturer’s software. Reconstructed CT and PET images were fused for quantitative data analysis. Regions of interest (ROIs) were drawn as guided by CT and quantitatively expressed as percent injected dose per gram of tissue (%ID/g).

The used ⁶⁴Cu-doped Cu(I)-GSH for PET imaging was synthesized and purified like the previous process with minor modification of adding radioactive ⁶⁴CuCl₂. In vivo mouse imaging was carried out on a Siemens Inveon PET-CT multimodality system (Siemens Medical Solutions, Knoxville, TN, USA) with spatial resolution of ~1.5 mm. ⁶⁴Cu-doped Cu(I)-GSH was administered to the BALB/c mouse (6–8 weeks) via iv-injection. PET images were collected immediately post injection (p.i.) for 1 h. The CT projections (360/rotation) were conducted with a power of 80 kV(p), current of 500 μA, exposure time of 145 ms, and binning size of 4. The first 1 h PET images were reconstructed into 20 frames of 180 s using a 3D Ordered Subsets Expectation Maximization (OSEM3D/MAP) algorithm. The CT reconstruction protocol used a down sample factor of 2, was set to interpolate bilinearly, and used a Shepp–Logan filter. The PET and CT images were coregistered in Inveon Acquisition Workplace (Siemens Medical Solutions, Knoxville, TN, USA) for analysis. Circular regions of interest (ROI) were drawn manually, encompassing the kidney and bladder in all planes containing the organs. The target activity was calculated as percentage injected dose per gram (%ID/g), which is defined as (activity (mCi/mL))/(total injected dose (mCi))/density (assuming density = 1 g/mL).

NHO volumes were measured in vivo in the Inveon PET-CT multimodality system (Siemens Medical Solutions, Inc.). Mice were anesthetized with a 2% isoflurane oxygen mixture. The parameters used were as follows: 360° rotation, 180 projections, 80 kV voltage, 500 μA current, and effective pixel size 36 μm. After 3D image reconstruction, NHO volume was quantified in the Inveon Research Workplace (Siemens Medical Solutions, Inc.) as previously described.^{20 (link),42 (link)}

Mouse PET/CT imaging was performed using a Siemens Inveon PET/CT Multi Modality system (Siemens Medical Solutions, Knoxville, TN) with effective spatial resolution of 1.4 mm at the center of field of view (FOV). All animals were fasted for 12 h prior to PET imaging. Each mouse received 140 μCi of 2-deoxy-2-(18F)fluoro-D-glucose (FDG) in 150 μL in saline intravenously via tail vain injection. The mice were placed on a heat pad before and during image acquisition. PET images were acquired 1h post-injection (p.i.), for 15 min, with animals under 2.5% isoflurane. PET images were reconstructed into a single frame using the 3D Ordered Subsets Expectation Maximization (OSEM3D/MAP) algorithm. CT images were acquired immediately after PET with the FOV centered at the shoulder of the mouse. CT projections (360 steps/rotation) were acquired with a power of 80 kVp, current of 500 μA, exposure time of 145 ms, binning of 4, and effective pixel size of 102 μm. The CT reconstruction protocol used a downsample factor of 2, was set to interpolate bilinearly, and used a Shepp-Logan filter. PET and CT images were co-registered in Inveon Acquisition Workplace (Siemens Medical Solutions) for analysis. Regions of interest (ROI) were drawn manually, encompassing the thyroid in all planes containing the organ. The target activity was calculated as percentage injected dose per gram.