Colony (focus) formation assay was performed to assess the cells’ ability to form colonies as described in Alvarez et al. with minor modifications [23 (link)]. Briefly, cells were seeded in 6-well plates at 500 cells per well in LHC-9 medium with 20 μM CdCl2 and cultured for a total of seven days. Cell foci were stained with crystal violet and images were captured using a digital scanner (V370 Photo, EPSON, Long Beach, CA, USA). Stained foci were solubilized and extracted using 10% acetic acid and absorbance was measured at 595 nm.
V370 photo
Manufactured by Epson
Sourced in United States
The Epson V370 Photo is a flatbed scanner designed for scanning 35mm film, slides, and medium format film. It features a resolution of up to 4800 dpi and can scan film in different sizes, including 35mm, 120, and 6x4.5 cm. The scanner connects via USB and is compatible with various operating systems.
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Lab products found in correlation
5 protocols using v370 photo
1
Colony Formation Assay for Cell Proliferation
2
Cell Colony Formation Assay
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A cell colony formation assay was carried out as described in previous study [61 (link)]. In detail, stable CYP27C1-knockdown A549 and H1975 cells and the corresponding control cells were seeded in 6-well plates with a density of 2000 cells per well. Cell colonies, formed after around 14 days, were fixed by methanol at room temperature for 10 min, followed by staining with crystal violet (0.5%, w/v) of for 10 min. The images of stained cell colonies were captured by a digital scanner (V370 Photo, EPSON, Long Beach, CA, USA). The stained cell colonies were then eluted by acetic acid (10%, v/v), and the absorbance of eluent was measured by a microplate photometer at 595 nm.
3
Quantifying Evans Blue Extravasation in Ovine Brain
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For the macroscopic analysis of the extravasation of Evans blue, the brain was coronally sliced and fixed in neutral-buffered formalin and a true color scan was conducted using a desktop photo scanner (V370 Photo, Epson, Suwa, Japan). Petechiae were counted in brains from six sheep. They were defined as blood spots <3 mm wide with indistinct edges. Those with defined edges, or following the distinct course of a blood vessel (characteristic of incomplete perfusion leaving unflushed blood vessels), were not counted. A 700 nm (far-red) scan of each brain section was done using a fluorescence scanner (Odyssey Fc, LI-COR, Lincoln, NE) for 2 min. Treated areas were delineated on each brain slice, as well as three untreated slices from each brain (slices 1-3 counting rostral to caudal), and the maximum far-red intensity for each area was recorded.
4
Digit Ratio Measurement Using High-Resolution Scanning
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Following common recommendations43 (link), both hands were scanned with a high-resolution scanner (Epson V370 Photo). To determine DR, we measured the lengths of the index and ring digits on both hands from basal crease to the fingertip using the computer software Adobe Photoshop® (Adobe Systems Inc., San Jose, USA).
Two independent raters measured the digit lengths. The DRs obtained from both raters had very high intraclass correlations (0.983 for the right hand DR and 0.977 for the left and DR). We averaged the raters’ measurements to conduct our analysis.
Although the DR studies often focus on the right hand data and a meta-analysis concludes that the right hand ratio is a better indicator, there are also numerous papers employing left hand ratios or even the means of both44 (link). We study the data for both hands separately but focus on interpreting the right hand DR results. Results gathered from right and left hand DRs are consistent.
Two independent raters measured the digit lengths. The DRs obtained from both raters had very high intraclass correlations (0.983 for the right hand DR and 0.977 for the left and DR). We averaged the raters’ measurements to conduct our analysis.
Although the DR studies often focus on the right hand data and a meta-analysis concludes that the right hand ratio is a better indicator, there are also numerous papers employing left hand ratios or even the means of both44 (link). We study the data for both hands separately but focus on interpreting the right hand DR results. Results gathered from right and left hand DRs are consistent.
5
Root System Morphometry Analysis
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At 10, 25, 40 and 50 DAG, all roots for each plant were scanned by a scanner (V370 Photo, Epson). Then, the length of each main root, L-type LR, S-type LR and secondary LR were extracted manually using ImageJ (US National Institutes of Health, Bethesda, USA). The diameter of the main roots, L-type LRs, S-type LRs and secondary LRs were measured using a microscope equipped with a digital camera (SMART-biomicroscopy, Chongqing Optec Instrument Co., Ltd).
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